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HRD1/SYVN1 Polyclonal antibody
HRD1/SYVN1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 13473-1-AP
|Positive WB detected in||HEK-293 cells, HepG2 cells, NIH/3T3 cells, Raji cells, mouse pancreas tissue, MDA-MB-453s cells, mouse ovary tissue, mouse kidney tissue, mouse stomach tissue, rat stomach tissue, HeLa cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13473-1-AP targets HRD1/SYVN1 in WB, IP-MS, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HRD1/SYVN1 fusion protein Ag4267|
|Full Name||synovial apoptosis inhibitor 1, synoviolin|
|Calculated molecular weight||617 aa, 68 kDa|
|Observed molecular weight||68-76 kDa|
|GenBank accession number||BC030530|
|Gene ID (NCBI)||84447|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).
|Product Specific Protocols|
|WB protocol for HRD1/SYVN1 antibody 13473-1-AP||Download protocol|
|IHC protocol for HRD1/SYVN1 antibody 13473-1-AP||Download protocol|
|IF protocol for HRD1/SYVN1 antibody 13473-1-AP||Download protocol|
|IP protocol for HRD1/SYVN1 antibody 13473-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Physiol Biochem
Endoplasmic Reticulum Stress Induces HRD1 to Protect Alveolar Type II Epithelial Cells from Apoptosis Induced by Cigarette Smoke Extract.
Acta Pharmacol Sin
Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.
J Biol Chem
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Exogenous H2S Induces Hrd1 S-sulfhydration and Prevents CD36 Translocation via VAMP3 Ubiquitylation in Diabetic Hearts.
J Biol Chem
Characterization of protein complexes of the endoplasmic reticulum associated degradation E3 ubiquitin ligase Hrd1.
SEL1L degradation intermediates stimulate cytosolic aggregation of polyglutamine-expanded protein.