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  • KD/KO Validated

HRD1/SYVN1 Polyclonal antibody

HRD1/SYVN1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP-MS, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13473-1-AP

Synonyms

HRD1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells, Raji cells, mouse pancreas tissue, MDA-MB-453s cells, mouse ovary tissue, mouse kidney tissue, mouse stomach tissue, rat stomach tissue, HeLa cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13473-1-AP targets HRD1/SYVN1 in WB, IP-MS, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HRD1/SYVN1 fusion protein Ag4267
Full Name synovial apoptosis inhibitor 1, synoviolin
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68-76 kDa
GenBank accession numberBC030530
Gene symbol SYVN1
Gene ID (NCBI) 84447
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

Protocols

Product Specific Protocols
WB protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IHC protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IF protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IP protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Physiol Biochem

Endoplasmic Reticulum Stress Induces HRD1 to Protect Alveolar Type II Epithelial Cells from Apoptosis Induced by Cigarette Smoke Extract.

Authors - Shuang-Xiang Tan
  • KD Validated
mouseWB,IHC

Acta Pharmacol Sin

Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.

Authors - Jian-Yi Dong
  • KD Validated
ratWB

J Biol Chem

The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.

Authors - Johnny J Saldate
  • KD Validated
mouseWB

Aging Dis

Exogenous H2S Induces Hrd1 S-sulfhydration and Prevents CD36 Translocation via VAMP3 Ubiquitylation in Diabetic Hearts.

Authors - Miao Yu
humanWB

J Biol Chem

Characterization of protein complexes of the endoplasmic reticulum associated degradation E3 ubiquitin ligase Hrd1.

Authors - Jiwon Hwang
  • KD Validated
humanWB

FEBS J

SEL1L degradation intermediates stimulate cytosolic aggregation of polyglutamine-expanded protein.

Authors - Tokuya Hattori