• Featured Product
  • KD/KO Validated

Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10795-1-AP

Synonyms

HI95, SES2, SESN2, SEST2, Sestrin2, Sestrin-2



Tested Applications

Positive WB detected inHEK-293T cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, rat liver tissue, K-562 cells, RAW264.7, human brain tissue, A2780 cells, A549 cells, human liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10795-1-AP targets Sestrin 2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Bovine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag1247
Full Name sestrin 2
Calculated molecular weight 54 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC013304
Gene symbol SESN2
Gene ID (NCBI) 83667
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).

Protocols

Product Specific Protocols
WB protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IHC protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IF protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IP protocol for Sestrin 2 antibody 10795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mar Drugs

Synergistic AML Cell Death Induction by Marine Cytotoxin (+)-1(R), 6(S), 1'(R), 6'(S), 11(R), 17(S)-Fistularin-3 and Bcl-2 Inhibitor Venetoclax.

Authors - Cristina Florean
ratWB

Am J Physiol Endocrinol Metab

Evidence for a Role for Sestrin1 in Mediating Leucine-Induced Activation of mTORC1 in Skeletal Muscle.

Authors - Dandan Xu
humanWB

Antioxid Redox Signal

Inhibitory Effect of Sestrin2 on Hepatic Stellate Cell Activation and Liver Fibrosis via Blocking Transforming Growth Factor-β Signaling.

Authors - Ji Hye Yang
humanWB

Cell Death Dis

PMID: 30952841

Authors - Hong Ri Jin
  • KO Validated
mouseWB

Am J Physiol Heart Circ Physiol

PMID: 31026186

Authors - Ruiting Li
  • KO Validated
humanWB

Free Radic Biol Med

Repositioning of niclosamide ethanolamine (NEN), an anthelmintic drug, for the treatment of lipotoxicity.

Authors - Jeong Su Park

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (04-05-2020)

This antibody works very well and very sensitive. I always get a specific and thick band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
Sestrin 2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10795-1-AP)