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Sestrin 2 Polyclonal antibody
Sestrin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
178
Cat no : 10795-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293T cells, HEK-293 cells, RAW264.7, A2780 cells, NIH/3T3 cells, human liver tissue, human brain tissue, rat liver tissue, A549 cells, K-562 cells, RAW 264.7 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human breast cancer tissue, human ovary tumor tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
10795-1-AP targets Sestrin 2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, Bovine, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Sestrin 2 fusion protein Ag1247 |
Full Name | sestrin 2 |
Calculated molecular weight | 54 kDa |
Observed molecular weight | 54-60 kDa |
GenBank accession number | BC013304 |
Gene symbol | SESN2 |
Gene ID (NCBI) | 83667 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).
Protocols
Product Specific Protocols | |
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WB protocol for Sestrin 2 antibody 10795-1-AP | Download protocol |
IHC protocol for Sestrin 2 antibody 10795-1-AP | Download protocol |
IF protocol for Sestrin 2 antibody 10795-1-AP | Download protocol |
IP protocol for Sestrin 2 antibody 10795-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine. | ||
Cell Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ashish (Verified Customer) (01-04-2022) | This is very good antibody for western blotting , even works good for IHC
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FH H (Verified Customer) (04-05-2020) | This antibody works very well and very sensitive. I always get a specific and thick band.
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