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Sestrin 2 Polyclonal antibody
Sestrin 2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 10795-1-AP
|Positive WB detected in||HEK-293T cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, rat liver tissue, K-562 cells, RAW264.7, human brain tissue, A2780 cells, A549 cells, human liver tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human breast cancer tissue, human ovary tumor tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10795-1-AP targets Sestrin 2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Bovine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Sestrin 2 fusion protein Ag1247|
|Full Name||sestrin 2|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||54-60 kDa|
|GenBank accession number||BC013304|
|Gene ID (NCBI)||83667|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).
|Product Specific Protocols|
|WB protocol for Sestrin 2 antibody 10795-1-AP||Download protocol|
|IHC protocol for Sestrin 2 antibody 10795-1-AP||Download protocol|
|IF protocol for Sestrin 2 antibody 10795-1-AP||Download protocol|
|IP protocol for Sestrin 2 antibody 10795-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Synergistic AML Cell Death Induction by Marine Cytotoxin (+)-1(R), 6(S), 1'(R), 6'(S), 11(R), 17(S)-Fistularin-3 and Bcl-2 Inhibitor Venetoclax.
Am J Physiol Endocrinol Metab
Evidence for a Role for Sestrin1 in Mediating Leucine-Induced Activation of mTORC1 in Skeletal Muscle.
Antioxid Redox Signal
Inhibitory Effect of Sestrin2 on Hepatic Stellate Cell Activation and Liver Fibrosis via Blocking Transforming Growth Factor-β Signaling.
Cell Death Dis
Am J Physiol Heart Circ Physiol
Free Radic Biol Med
Repositioning of niclosamide ethanolamine (NEN), an anthelmintic drug, for the treatment of lipotoxicity.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
H (Verified Customer) (04-05-2020)
This antibody works very well and very sensitive. I always get a specific and thick band.