Sestrin 2 Polyclonal antibody

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  WB analysis using 10795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10795-1-AP

  WB result of Sestrin antibody (10795-1-AP, 1:2000) with si-Control and si-Sestrin transfected HEK293 cells.

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  WB analysis using 10795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10795-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10795-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 10795-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of human liver using 10795-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10795-1-AP

  IP result of anti-Sestrin 2 (IP:10795-1-AP, 3ug; Detection:10795-1-AP 1:1000) with K-562 cells lysate 4000ug.

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  IHC staining of human breast cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of human colon cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse kidney using 10795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Sestrin 2 antibody (10795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10795-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (10795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

Sestrin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 10795-1-AP

Publications(210)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

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Tested Applications

• Positive WB detected in: HEK-293 cells, human liver tissue, human brain tissue, NIH/3T3 cells, rat liver tissue, HeLa cells, K-562 cells, mouse kidney tissue, A2780 cells, RAW 264.7 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 56 publications below
• WB: See 189 publications below
• IHC: See 19 publications below
• IF: See 27 publications below
• IP: See 12 publications below
• FC: See 2 publications below
• CoIP: See 3 publications below

Product Information

10795-1-AP targets Sestrin 2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sestrin 2 fusion protein Ag1247
• Full Name: sestrin 2
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC013304
• Gene Symbol: SESN2/Sestrin 2
• Gene ID (NCBI): 83667
• RRID: AB_2185480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P58004
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).

Publications

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
mouse	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
human	WB	Nature; Stem cell function and stress response are controlled by protein synthesis.; Authors - Sandra Blanco
mouse	WB	Science; Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.; Authors - Andrew L Cangelosi
human	WB	Nature; SZT2 dictates GATOR control of mTORC1 signalling.; Authors - Min Peng
mouse	WB	Cell; Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling.; Authors - Min Peng ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ashish (Verified Customer) (01-04-2022)

This is very good antibody for western blotting , even works good for IHC

• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HeK293T , HeLA

FH

H (Verified Customer) (04-05-2020)

This antibody works very well and very sensitive. I always get a specific and thick band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

Sestrin 2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10795-1-AP)