Sestrin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Sestrin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

178

Reviews (2)

Cat no : 10795-1-AP

Synonyms

HI95, SES2, SESN2, SEST2, Sestrin 2, Sestrin2, Sestrin-2

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Validation Data Gallery

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  WB analysis using 10795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10795-1-AP

  WB result of Sestrin antibody (10795-1-AP, 1:2000) with si-Control and si-Sestrin transfected HEK293 cells.

• 

  WB analysis of human brain using 10795-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10795-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10795-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10795-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10795-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of A549 cells using 10795-1-AP

  A549 cells (shcontrol and shRNA of SESN2) were subjected to SDS PAGE followed by western blot with 10795-1-AP (SESN2 antibody) at dilution of 1:1000. .

• 

  WB analysis of A2780 using 10795-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10795-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10795-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 10795-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10795-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 10795-1-AP

  IP Result of anti-Sestrin 2 (IP:10795-1-AP, 3ug; Detection:10795-1-AP 1:1000) with K-562 cells lysate 4000ug.

• 

  IHC staining of human breast cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:50 (under 25x lens).

• 

  IHC staining of human breast cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 10795-1-AP

  Immunofluorescent analysis of Hela cells, using SESN2 antibody 10795-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, HEK-293 cells, RAW264.7, A2780 cells, NIH/3T3 cells, human liver tissue, human brain tissue, rat liver tissue, A549 cells, K-562 cells, RAW 264.7 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 49 publications below
• WB: See 161 publications below
• IHC: See 13 publications below
• IF: See 20 publications below
• IP: See 10 publications below
• FC: See 2 publications below
• CoIP: See 2 publications below

Product Information

10795-1-AP targets Sestrin 2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Bovine, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sestrin 2 fusion protein Ag1247
• Full Name: sestrin 2
• Calculated molecular weight: 54 kDa
• Observed molecular weight: 54-60 kDa
• GenBank accession number: BC013304
• Gene symbol: SESN2
• Gene ID (NCBI): 83667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).

Publications

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
mouse	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
human	WB	Nature; Stem cell function and stress response are controlled by protein synthesis.; Authors - Sandra Blanco
mouse	WB	Science; Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.; Authors - Andrew L Cangelosi
human	WB	Nature; SZT2 dictates GATOR control of mTORC1 signalling.; Authors - Min Peng
mouse	WB	Cell; Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling.; Authors - Min Peng ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ashish (Verified Customer) (01-04-2022)

This is very good antibody for western blotting , even works good for IHC

• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HeK293T , HeLA

FH

H (Verified Customer) (04-05-2020)

This antibody works very well and very sensitive. I always get a specific and thick band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

Sestrin 2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10795-1-AP)

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