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Sestrin 2 Polyclonal antibody
Sestrin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (2)
WB, IP, IHC, IF, FC, CoIP, ELISA
Cat no : 10795-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293T cells, HEK-293 cells, RAW264.7, A2780 cells, NIH/3T3 cells, human liver tissue, human brain tissue, rat liver tissue, A549 cells, K-562 cells, RAW 264.7 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human breast cancer tissue, human ovary tumor tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10795-1-AP targets Sestrin 2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Bovine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Sestrin 2 fusion protein Ag1247|
|Full Name||sestrin 2|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||54-60 kDa|
|GenBank accession number||BC013304|
|Gene ID (NCBI)||83667|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).
|Product Specific Protocols|
|WB protocol for Sestrin 2 antibody 10795-1-AP||Download protocol|
|IHC protocol for Sestrin 2 antibody 10795-1-AP||Download protocol|
|IF protocol for Sestrin 2 antibody 10795-1-AP||Download protocol|
|IP protocol for Sestrin 2 antibody 10795-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Systematic quantitative analysis of ribosome inventory during nutrient stress.
SAR1B senses leucine levels to regulate mTORC1 signalling.
Stem cell function and stress response are controlled by protein synthesis.
Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.
SZT2 dictates GATOR control of mTORC1 signalling.
Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ashish (Verified Customer) (01-04-2022)
This is very good antibody for western blotting , even works good for IHC
H (Verified Customer) (04-05-2020)
This antibody works very well and very sensitive. I always get a specific and thick band.