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  • KD/KO Validated

Sestrin 2 Monoclonal antibody

Sestrin 2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat no : 66297-1-Ig

Synonyms

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2



Tested Applications

Positive WB detected inHeLa cells, HEK-293T
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66297-1-Ig targets Sestrin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Cited Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag20638
Full Name sestrin 2
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC013304
Gene symbol SESN2
Gene ID (NCBI) 83667
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Protocols

Product Specific Protocols
WB protocol for Sestrin 2 antibody 66297-1-IgDownload protocol
IHC protocol for Sestrin 2 antibody 66297-1-IgDownload protocol
IF protocol for Sestrin 2 antibody 66297-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Pharm Pharmacol

Effect of atorvastatin on Aβ1-42 -induced alteration of SESN2, SIRT1, LC3II and TPP1 protein expressions in neuronal cell cultures.

Authors - Hande Çelik
humanWB

Am J Reprod Immunol

Sestrin2 alleviates palmitate-induced endoplasmic reticulum stress, apoptosis, and defective invasion of human trophoblast cells.

Authors - Solji Lee
  • KD Validated
pigWB

Am J Physiol Endocrinol Metab

Prematurity blunts the insulin- and amino acid-induced stimulation of translation initiation and protein synthesis in skeletal muscle of neonatal pigs.

Authors - Marko Rudar
pigWB, IP

J Nutr

Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.

Authors - Agus Suryawan
humanWB,IHC

Osteoarthritis Cartilage

Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.

Authors - T Shen
human,mouseWB,IF

Cell Biol Toxicol

Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy.

Authors - Yiming Jiang
  • KD Validated