|Positive WB detected in||HeLa cells, HEK-293T|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66297-1-Ig targets Sestrin 2 in WB, IHC, IF,ELISA applications and shows reactivity with human, pig, rat samples.
|Tested Reactivity||human, pig, rat|
|Cited Reactivity||human, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Sestrin 2 fusion protein Ag20638|
|Full Name||sestrin 2|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC013304|
|Gene ID (NCBI)||83667|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).
Am J Reprod Immunol
Sestrin2 alleviates palmitate-induced endoplasmic reticulum stress, apoptosis, and defective invasion of human trophoblast cells.
Am J Physiol Endocrinol Metab
Prematurity blunts the insulin- and amino acid-induced stimulation of translation initiation and protein synthesis in skeletal muscle of neonatal pigs.
Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.