TBRG4 Polyclonal antibody

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  WB analysis of HepG2 using 16245-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16245-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16245-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16245-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16245-1-AP

  IP result of anti-TBRG4 (IP:16245-1-AP, 4ug; Detection:16245-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human liver cancer using 16245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS cells using 16245-1-AP

  IF result of anti-TBRG4 (16245-1-AP) with U2OS cells.

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  IF Staining of COS7 cells using 16245-1-AP

  IF result of 16245-1-AP (anti-KD4) in COS7 cell by Dr. Nancy Kedersha. COS7 cells, fixed with 4% paraformaldehyde, post/fied and permeabilized with methanol, and stained with PTG anti-FAST KD4 antibody(16245-1-AP) in green, and human anti-mito autoimune sera from Immunovision in red. Nuclei are stained blue using Hoechst dye.

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  IF Staining of HeLa using 16245-1-AP

  Immunofluorescent analysis of Hela cells, using TBRG4 antibody 16245-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

TBRG4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16245-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CPR2, FASTKD4, KIAA0948, Protein TBRG4, TBRG4

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : N/A

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16245-1-AP targets TBRG4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9176

  Product name: Recombinant human TBRG4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 280-631 aa of BC017235

  Sequence: SYHLVQKPFSLTKDVLLDVAYAYGKLSFHQTQVSQRLATDLLSLMPSLTSGEVAHCAKSFALLKWLSLPLFEAFAQHVLNRAQDITLPHLCSVLLAFARLNFHPDQEDQFFSLVHEKLGSELPGLEPALQVDLVWALCVLQQAREAELQAVLHPEFHIQFLGGKSQKDQNTFQKLLHINATALLEYPEYSGPLLPASAVAPGPSALDRKVTPLQKELQETLKGLLGSADKGSLEVATQYGWVLDAEVLLDSDGEFLPVRDFVAPHLAQPTGSQSPPPGSKRLAFLRWEFPNFNSRSKDLLGRFVLARRHIVAAGFLIVDVPFYEWLELKSEWQKGAYLKDKMRKAVAEELAK

• Full Name: transforming growth factor beta regulator 4
• Calculated Molecular Weight: 631 aa, 71 kDa
• Observed Molecular Weight: 60 kDa, 71 kDa
• GenBank Accession Number: BC017235
• Gene Symbol: TBRG4
• Gene ID (NCBI): 9238
• RRID: AB_2271487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969Z0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	PLoS Genet; The FASTK family proteins fine-tune mitochondrial RNA processing; Authors - Akira Ohkubo ; KO Validated
human	WB,IHC,IF,CoIP	BMC Cancer; High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma; Authors - Shanchun Tao
human	WB	Life Sci Alliance; Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins.; Authors - Claudio Asencio
human	WB,IP	Nucleic Acids Res; The Vsr-like protein FASTKD4 regulates the stability and polyadenylation of the MT-ND3 mRNA; Authors - Xuan Yang ; KO Validated
human	WB,IP,IF	Spine J; TBRG4 regulates ubiquitination of Beclin1 and participates in autophagy pathway to inhibit intervertebral disc degeneration; Authors - Xilong Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Erik (Verified Customer) (02-24-2021)

The antibody used at 1:500 dilution produces a clean and strong band at the expected height for the protein

• Applications: Western Blot
• Cell Tissue Type: PANC1 and MIAPACA2 cell lines

TBRG4 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:16245-1-AP)