TBRG4 Polyclonal antibody

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• TBRG4 Polyclonal antibody (16245-1-AP)

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• KD/KO Validated

TBRG4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16245-1-AP Copied!

Publications (5)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CPR2, FAST kinase domain-containing protein 4, FASTKD4, KIAA0948, Protein TBRG4

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  WB analysis of HepG2 using 16245-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 16245-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 16245-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16245-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 16245-1-AP

  IP result of anti-TBRG4 (IP:16245-1-AP, 4ug; Detection:16245-1-AP 1:500) with HepG2 cells lysate 1600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 16245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 16245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS cells using 16245-1-AP

  IF result of anti-TBRG4 (16245-1-AP) with U2OS cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of COS7 cells using 16245-1-AP

  IF result of 16245-1-AP (anti-KD4) in COS7 cell by Dr. Nancy Kedersha. COS7 cells, fixed with 4% paraformaldehyde, post/fied and permeabilized with methanol, and stained with PTG anti-FAST KD4 antibody(16245-1-AP) in green, and human anti-mito autoimune sera from Immunovision in red. Nuclei are stained blue using Hoechst dye.

• 

  IF Staining of HeLa using 16245-1-AP

  Immunofluorescent analysis of Hela cells, using TBRG4 antibody 16245-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : N/A

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16245-1-AP targets TBRG4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9176

  Product name: Recombinant human TBRG4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 280-631 aa of BC017235

  Sequence: SYHLVQKPFSLTKDVLLDVAYAYGKLSFHQTQVSQRLATDLLSLMPSLTSGEVAHCAKSFALLKWLSLPLFEAFAQHVLNRAQDITLPHLCSVLLAFARLNFHPDQEDQFFSLVHEKLGSELPGLEPALQVDLVWALCVLQQAREAELQAVLHPEFHIQFLGGKSQKDQNTFQKLLHINATALLEYPEYSGPLLPASAVAPGPSALDRKVTPLQKELQETLKGLLGSADKGSLEVATQYGWVLDAEVLLDSDGEFLPVRDFVAPHLAQPTGSQSPPPGSKRLAFLRWEFPNFNSRSKDLLGRFVLARRHIVAAGFLIVDVPFYEWLELKSEWQKGAYLKDKMRKAVAEELAK

• Full Name: transforming growth factor beta regulator 4
• Calculated Molecular Weight: 631 aa, 71 kDa
• Observed Molecular Weight: 60 kDa, 71 kDa
• GenBank Accession Number: BC017235
• Gene Symbol: TBRG4
• Gene ID (NCBI): 9238
• RRID: AB_2271487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969Z0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing.

Publications

What published studies show

The anti-TBRG4 antibody (Cat# 16245-1-AP) has 5 total citations. Publications appear in Nucleic Acids Research, Spine Journal, BMC Cancer, PLoS Genetics, and Life Science Alliance. Research fields span mitochondrial RNA processing, autophagy regulation, hepatocellular carcinoma and ferroptosis, FASTK family protein function, and nucleic acid–protein interaction studies. Applications include WB, IP, IF, IHC, and CoIP, all in human samples.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Nucleic Acids Res; The Vsr-like protein FASTKD4 regulates the stability and polyadenylation of the MT-ND3 mRNA; Authors - Xuan Yang ; KO Validated
human	WB,IP,IF	Spine J; TBRG4 regulates ubiquitination of Beclin1 and participates in autophagy pathway to inhibit intervertebral disc degeneration; Authors - Xilong Cui
human	WB,IHC,IF,CoIP	BMC Cancer; High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma; Authors - Shanchun Tao
human	WB	PLoS Genet; The FASTK family proteins fine-tune mitochondrial RNA processing; Authors - Akira Ohkubo ; KO Validated
human	WB	Life Sci Alliance; Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins.; Authors - Claudio Asencio

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The single review for this product (rated 4/5) reports successful use in Western Blot on PANC1 and MIAPACA2 cell lines. The reviewer noted that at a 1:500 dilution, the antibody produced a clean, strong band at the expected molecular weight, indicating good specificity and sensitivity for the target protein.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Erik (Verified Customer) (02-24-2021)

The antibody used at 1:500 dilution produces a clean and strong band at the expected height for the protein

• Applications: Western Blot
• Cell Tissue Type: PANC1 and MIAPACA2 cell lines

TBRG4 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:16245-1-AP)