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  • KD/KO Validated

TDO2 Polyclonal antibody

TDO2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15880-1-AP

Synonyms

TDO, TDO2, TO, TRPO, Tryptamin 2,3 dioxygenase, tryptophan 2,3 dioxygenase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15880-1-AP targets TDO2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TDO2 fusion protein Ag8642
Full Name tryptophan 2,3-dioxygenase
Calculated molecular weight 406 aa, 48 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC005355
Gene symbol TDO2
Gene ID (NCBI) 6999
RRIDAB_2827610
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TDO2 (tryptophan 2,3-dioxygenase), IDO1 (indoleamine 2,3-dioxygenase 1) and IDO2 are three enzymes that catalyze the amino acid tryptophan into kynurenine in the kynurenine pathway (PMID: 23090118, 28469468). TDO2 plays an important role in neurological diseases such as Alzheimer's disease, Parkinson's disease and autism. Overexpression of TDO2 promoted tumor cell survival and was correlated with tumor grade and poor prognosis in triple negative breast cancer, brain tumors and esophageal squamous cell carcinoma. TDO2 may be a potential therapeutic target in some cancers. (PMID: 21976023, 30134247)

Protocols

Product Specific Protocols
WB protocol for TDO2 antibody 15880-1-APDownload protocol
IHC protocol for TDO2 antibody 15880-1-APDownload protocol
IF protocol for TDO2 antibody 15880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB

Cancer Cell

Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.

Authors - Krishna S Tummala
  • KD Validated
humanWB

Nat Commun

Engineered MED12 mutations drive leiomyoma-like transcriptional and metabolic programs by altering the 3D genome compartmentalization

Authors - Kadir Buyukcelebi
human,ratWB,IHC,IF

Br J Pharmacol

Tryptophan 2,3-dioxygenase 2 plays a key role in regulating the activation of fibroblast-like synoviocytes in autoimmune arthritis.

Authors - Yan Chang
  • KD Validated
mouseWB

Front Immunol

DSS-induced colitis activates the kynurenine pathway in serum and brain by affecting IDO-1 and gut microbiota

Authors - Li-Ping Zhao
humanWB,IHC

Fertil Steril

Tryptophan catabolism is dysregulated in leiomyomas.

Authors - Tsai-Der Chuang
mouseWB

J Med Chem

Discovery of 1-(Hetero)aryl-β-carboline Derivatives as IDO1/TDO Dual Inhibitors with Antidepressant Activity

Authors - Yu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hala (Verified Customer) (03-10-2021)

works veryyy gooddd

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000 - 1/2000
  • Cell Tissue Type: hepatoblastoma cells transduced with lentivirus expressing TDO2
FH

Hala (Verified Customer) (12-15-2020)

so efficient

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/750 - 1/1000
  • Cell Tissue Type: liver cells transduced with lentivirus expressing TDO2
TDO2 Antibody Western Blot validation (1/750 - 1/1000 dilution) in liver cells transduced with lentivirus expressing TDO2 (Cat no:15880-1-AP)