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  • KD/KO Validated

TDO2 Polyclonal antibody

TDO2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15880-1-AP

Synonyms

TDO, TDO2, TO, TPH2, TRPO, Tryptamin 2,3 dioxygenase, tryptophan 2,3 dioxygenase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase



Tested Applications

Positive WB detected inrat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

15880-1-AP targets TDO2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TDO2 fusion protein Ag8642
Full Name tryptophan 2,3-dioxygenase
Calculated molecular weight 406 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC005355
Gene symbol TDO2
Gene ID (NCBI) 6999
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TDO2 (tryptophan 2,3-dioxygenase), IDO1 (indoleamine 2,3-dioxygenase 1) and IDO2 are three enzymes that catalyze the amino acid tryptophan into kynurenine in the kynurenine pathway (PMID: 23090118, 28469468). TDO2 plays an important role in neurological diseases such as Alzheimer's disease, Parkinson's disease and autism. Overexpression of TDO2 promoted tumor cell survival and was correlated with tumor grade and poor prognosis in triple negative breast cancer, brain tumors and esophageal squamous cell carcinoma. TDO2 may be a potential therapeutic target in some cancers. (PMID: 21976023, 30134247)

Protocols

Product Specific Protocols
WB protocol for TDO2 antibody 15880-1-APDownload protocol
IHC protocol for TDO2 antibody 15880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Metab

Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation.

Authors - Curtis C Hughey
humanWB,IHC

Signal Transduct Target Ther

The proatherosclerotic function of indoleamine 2, 3-dioxygenase 1 in the developmental stage of atherosclerosis.

Authors - Heng Liang
mouseWB

Cell Rep

Pharmacological Wnt ligand inhibition overcomes key tumor-mediated resistance pathways to anti-PD-1 immunotherapy.

Authors - Nicholas C DeVito
mouseWB,IHC,FC,IF

Transgenic Res

A comprehensive analysis of TDO2 expression in immune cells and characterization of immune cell phenotype in TDO2 knockout mice.

Authors - Susu Li
ratWB

Chem Res Toxicol

Tryptophan Pathway-Targeted Metabolomics Study on the Mechanism and Intervention of Cisplatin-Induced Acute Kidney Injury in Rats.

Authors - Bei Tan
humanWB

Toxicol Appl Pharmacol

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Authors - Azubuike Peter Ebokaiwe

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hala (Verified Customer) (03-10-2021)

works veryyy gooddd

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000 - 1/2000
  • Cell Tissue Type: hepatoblastoma cells transduced with lentivirus expressing TDO2
FH

Hala (Verified Customer) (12-15-2020)

so efficient

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/750 - 1/1000
  • Cell Tissue Type: liver cells transduced with lentivirus expressing TDO2
TDO2 Antibody Western Blot validation (1/750 - 1/1000 dilution) in liver cells transduced with lentivirus expressing TDO2 (Cat no:15880-1-AP)