TDO2 Polyclonal antibody

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  WB analysis using 15880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15880-1-AP (TDO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15880-1-AP (TDO2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15880-1-AP (TDO2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15880-1-AP (TDO2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15880-1-AP (TDO2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15880-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TDO2 antibody (15880-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

TDO2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15880-1-AP

Publications(41)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Tryptamin 2,3 dioxygenase, TRPO, TPH2, TO, TDO

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 34 publications below
• IHC: See 13 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Product Information

15880-1-AP targets TDO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TDO2 fusion protein Ag8642
• Full Name: tryptophan 2,3-dioxygenase
• Calculated Molecular Weight: 406 aa, 48 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC005355
• Gene Symbol: TDO2
• Gene ID (NCBI): 6999
• RRID: AB_2827610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48775
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TDO2 (tryptophan 2,3-dioxygenase), IDO1 (indoleamine 2,3-dioxygenase 1) and IDO2 are three enzymes that catalyze the amino acid tryptophan into kynurenine in the kynurenine pathway (PMID: 23090118, 28469468). TDO2 plays an important role in neurological diseases such as Alzheimer's disease, Parkinson's disease and autism. Overexpression of TDO2 promoted tumor cell survival and was correlated with tumor grade and poor prognosis in triple negative breast cancer, brain tumors and esophageal squamous cell carcinoma. TDO2 may be a potential therapeutic target in some cancers. (PMID: 21976023, 30134247)

Publications

Species	Application	Title
human, mouse	WB	Cancer Cell; Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.; Authors - Krishna S Tummala ; KD Validated
human	WB	Nat Commun; Engineered MED12 mutations drive leiomyoma-like transcriptional and metabolic programs by altering the 3D genome compartmentalization; Authors - Kadir Buyukcelebi
human	WB	ACS Appl Mater Interfaces; Reprogrammed IDO-Induced Immunosuppressive Microenvironment Synergizes with Immunogenic Magnetothermodynamics for Improved Cancer Therapy; Authors - Xun Wang
human,rat	WB,IHC,IF	Br J Pharmacol; Tryptophan 2,3-dioxygenase 2 plays a key role in regulating the activation of fibroblast-like synoviocytes in autoimmune arthritis.; Authors - Yan Chang ; KD Validated
mouse	WB	Front Immunol; DSS-induced colitis activates the kynurenine pathway in serum and brain by affecting IDO-1 and gut microbiota; Authors - Li-Ping Zhao
human	WB,IHC	Fertil Steril; Tryptophan catabolism is dysregulated in leiomyomas.; Authors - Tsai-Der Chuang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hala (Verified Customer) (03-10-2021)

works veryyy gooddd

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000 - 1/2000
• Cell Tissue Type: hepatoblastoma cells transduced with lentivirus expressing TDO2

FH

Hala (Verified Customer) (12-15-2020)

so efficient

• Applications: Western Blot
• Primary Antibody Dilution: 1/750 - 1/1000
• Cell Tissue Type: liver cells transduced with lentivirus expressing TDO2

TDO2 Antibody Western Blot validation (1/750 - 1/1000 dilution) in liver cells transduced with lentivirus expressing TDO2 (Cat no:15880-1-AP)