TDP-43 Recombinant antibody

• Featured Product
• KD/KO Validated

TDP-43 Recombinant Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

16A22

Cat no : 80002-1-RR

Synonyms

ALS10, TAR DNA binding protein 43, TARDBP, tdp 43, TDP43, TDP-43

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Validation Data Gallery

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  WB analysis of HeLa using 80002-1-RR

  Various cell lysates were subjected to SDS PAGE followed by western blot with 80002-1-RR (TDP-43 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 80002-1-RR

  HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80002-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 80002-1-RR

  WB result of TDP-43 antibody (80002-1-RR; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

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  IP experiment of HAP1 using 80002-1-RR

  HAP1 lysates prepared and IP of TARDBP performed using 2.0 μg of 80002-1-RR coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of mouse brain using 80002-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80002-1-RR (TDP-43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HAP1 using 80002-1-RR

  HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80002-1-RR at 1:200 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IF Staining of SH-SY5Y using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

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  IF Staining of HeLa using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of rat brain using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 80002-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 80002-1-RR (TDP-43 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin.

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  FC experiment of HeLa using 80002-1-RR

  1X10^6 HeLa cells were intracellularly stained with 0.20ug Anti-Human TDP-43 (80002-1-RR, Clone:16A22) (red) or 0.20 ug control antibody (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HeLa cells, HAP1 cells, K-562 cells, Neuro-2a cells, C6 cells
• Positive IP detected in: HAP1 cells
• Positive IF detected in: SH-SY5Y cells, rat brain tissue, HAP1 cells, HeLa cells, HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

80002-1-RR targets TDP-43 in WB, IP, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TDP-43 fusion protein Ag1231
• Full Name: TAR DNA binding protein
• Calculated molecular weight: 43 kDa
• Observed molecular weight: 43 kDa
• GenBank accession number: BC001487
• Gene symbol: TARDBP
• Gene ID (NCBI): 23435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)

80002-1-RR antibody works well in IF experiment.