TDP-43 (for IF/FC) Recombinant antibody

TDP-43 (for IF/FC) Recombinant Antibody for FC, IF,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

16A22

Cat no : 80002-1-RR

Synonyms

TAR DNA binding protein 43, TARDBP, TDP43, TDP-43



Tested Applications

Positive IF detected inSH-SY5Y cells, A431 cells, rat brain tissue, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

80002-1-RR targets TDP-43 (for IF/FC) in IF, FC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TDP-43 (for IF/FC) fusion protein Ag1231
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)

80002-1-RR antibody works well in IF experiment.

Protocols

Product Specific Protocols
WB protocol for TDP-43 (for IF/FC) antibody 80002-1-RRDownload protocol
IHC protocol for TDP-43 (for IF/FC) antibody 80002-1-RRDownload protocol
IF protocol for TDP-43 (for IF/FC) antibody 80002-1-RRDownload protocol
FC protocol for TDP-43 (for IF/FC) antibody 80002-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols