CoraLite®488-conjugated TDP-43 (for IF/FC) Recombinant antibody

TDP-43 (for IF/FC) Recombinant Antibody for FC, IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF, FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

16A22

Cat no : CL488-80002

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43



Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 80002-1-RR

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-80002 targets TDP-43 (for IF/FC) in IF, FC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TDP-43 (for IF/FC) fusion protein Ag1231
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).

Protocols

Product Specific Protocols
IF protocol for CL488 TDP-43 (for IF/FC) antibody CL488-80002Download protocol
FC protocol for CL488 TDP-43 (for IF/FC) antibody CL488-80002Download protocol
Standard Protocols
Click here to view our Standard Protocols