CoraLite® Plus 488-conjugated TDP-43 (for IF/FC) Recombinant antibody

TDP-43 (for IF/FC) Recombinant Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

16A22

Cat no : CL488-80002

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 (for IF/FC)



Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-80002 targets TDP-43 (for IF/FC) in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TDP-43 (for IF/FC) fusion protein Ag1231
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
RRIDAB_2883403
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TDP-43 (for IF/FC) antibody CL488-80002Download protocol
Standard Protocols
Click here to view our Standard Protocols