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TEAD4 Polyclonal antibody
TEAD4 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 12418-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | BGC-823 cells, MCF-7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 6 publications below |
CoIP | See 2 publications below |
Product Information
12418-1-AP targets TEAD4 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TEAD4 fusion protein Ag3059 |
Full Name | TEA domain family member 4 |
Calculated molecular weight | 434 aa, 48 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC015497 |
Gene symbol | TEAD4 |
Gene ID (NCBI) | 7004 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TEAD4 belongs to a protein family that share the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins , a TBP-associated factor and a limiting transcriptional intermediary facotrs. TEAD4 also preforms an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but molecular weight of TEAD4 is detected about 65 kDa as same as the result from the paper (PMID: 28051067).
Protocols
Product Specific Protocols | |
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WB protocol for TEAD4 antibody 12418-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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Carcinogenesis A yes-associated protein 1-Notch1 positive feedback loop promotes breast cancer lung metastasis by attenuating the Bone morphogenetic protein 4-SMAD family member 1/5 signaling | ||
J Cell Mol Med NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.
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Mol Carcinog YAP/TEAD4-induced KIF4A contributes to the progression and worse prognosis of esophageal squamous cell carcinoma. | ||
J Oral Pathol Med Systematic screening identifies a TEAD4-S100A13 axis modulating cisplatin sensitivity of oral squamous cell carcinoma cells.
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J Exp Clin Cancer Res TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer.
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