TEAD4 Polyclonal antibody

TEAD4 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12418-1-AP

Synonyms

EFTR 2, hRTEF 1B, RTEF1, TCF13L1, TEA domain family member 4, TEAD 4, TEAD4, TEF 3, TEF3, TEFR 1, Transcription factor 13 like 1, Transcription factor RTEF 1

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Validation Data Gallery

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  WB analysis using 12418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12418-1-AP (TEAD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12418-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12418-1-AP (TEAD4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 12418-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 12418-1-AP (TEAD4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BGC-823 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• CoIP: See 2 publications below

Product Information

12418-1-AP targets TEAD4 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEAD4 fusion protein Ag3059
• Full Name: TEA domain family member 4
• Calculated molecular weight: 434 aa, 48 kDa
• Observed molecular weight: 65 kDa
• GenBank accession number: BC015497
• Gene symbol: TEAD4
• Gene ID (NCBI): 7004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TEAD4 belongs to a protein family that share the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins , a TBP-associated factor and a limiting transcriptional intermediary facotrs. TEAD4 also preforms an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but molecular weight of TEAD4 is detected about 65 kDa as same as the result from the paper (PMID: 28051067).

Publications

Species	Application	Title
human	CoIP	J Cell Mol Med; PMID: 31559693; Authors - Yangyang Yao
human	WB	J Oral Pathol Med; Systematic screening identifies a TEAD4-S100A13 axis modulating cisplatin sensitivity of oral squamous cell carcinoma cells.; Authors - Ruicen Li ; KD Validated
human	WB	Mol Carcinog; YAP/TEAD4-induced KIF4A contributes to the progression and worse prognosis of esophageal squamous cell carcinoma.; Authors - Ya Li
human	WB,CoIP	J Cell Mol Med; NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.; Authors - Bowen Hu ; KD Validated
mouse	WB	J Cell Biol; Thyroid hormone regulates muscle fiber type conversion via miR-133a1.; Authors - Duo Zhang