TEAD4 Polyclonal antibody

• Featured Product
• KD/KO Validated

TEAD4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 12418-1-AP

Synonyms

EFTR 2, hRTEF 1B, RTEF1, TCF13L1, TEA domain family member 4, TEAD 4, TEAD4, TEF 3, TEF3, TEFR 1, Transcription factor 13 like 1, Transcription factor RTEF 1

---

Validation Data Gallery

• 

  WB analysis using 12418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12418-1-AP (TEAD4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: BGC-823 cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Product Information

12418-1-AP targets TEAD4 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEAD4 fusion protein Ag3059
• Full Name: TEA domain family member 4
• Calculated molecular weight: 434 aa, 48 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC015497
• Gene symbol: TEAD4
• Gene ID (NCBI): 7004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD4 belongs to a protein family that share the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins , a TBP-associated factor and a limiting transcriptional intermediary facotrs. TEAD4 also preforms an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but molecular weight of TEAD4 is detected about 50-55 kDa.

Publications

Species	Application	Title
mouse	WB	J Cell Biol; Thyroid hormone regulates muscle fiber type conversion via miR-133a1.; Authors - Duo Zhang
human	WB	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen
human	CoIP	J Cell Mol Med; PMID: 31559693; Authors - Yangyang Yao
human	WB	Carcinogenesis; A yes-associated protein 1-Notch1 positive feedback loop promotes breast cancer lung metastasis by attenuating the Bone morphogenetic protein 4-SMAD family member 1/5 signaling; Authors - Lin Zhao
human	WB,CoIP	J Cell Mol Med; NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.; Authors - Bowen Hu ; KD Validated
human	WB	Mol Carcinog; YAP/TEAD4-induced KIF4A contributes to the progression and worse prognosis of esophageal squamous cell carcinoma.; Authors - Ya Li