TEAD4 Polyclonal antibody

• 

  WB analysis using 12418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12418-1-AP (TEAD4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

TEAD4 Polyclonal Antibody for WB, ELISA

Cat No. 12418-1-AP

Publications(27)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, CoIP, ChIP, ELISA

Synonyms

EFTR 2, hRTEF 1B, RTEF1, TCF13L1, TEA domain family member 4

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Tested Applications

• Positive WB detected in: BGC-823 cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Product Information

12418-1-AP targets TEAD4 in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEAD4 fusion protein Ag3059
• Full Name: TEA domain family member 4
• Calculated Molecular Weight: 434 aa, 48 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC015497
• Gene Symbol: TEAD4
• Gene ID (NCBI): 7004
• RRID: AB_2203074
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15561
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD4 belongs to a protein family that share the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins , a TBP-associated factor and a limiting transcriptional intermediary facotrs. TEAD4 also preforms an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but molecular weight of TEAD4 is detected about 50-55 kDa.

Publications

Species	Application	Title
mouse	WB	J Cell Biol; Thyroid hormone regulates muscle fiber type conversion via miR-133a1.; Authors - Duo Zhang
human	WB	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen
human	WB	Br J Pharmacol; An agonist of the adenosine A2A receptor, CGS21680, promotes corneal epithelial wound healing via the YAP signalling pathway; Authors - Qiuqin Sun
mouse,human	WB,IF	Clin Sci (Lond); IGFBP7 promotes endothelial cell repair in the recovery phase of acute lung injury; Authors - Rui He
human	CoIP	J Cell Mol Med; PMID: 31559693; Authors - Yangyang Yao
human	ChIP	Carcinogenesis; Integrative functional screen of genomic loci uncovers CCND2 and its genetic regulatory mechanism in colorectal cancer development; Authors - Bin Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Pramod Bahadur (Verified Customer) (03-05-2024)

We used this antibody for PC3 cell lines with vector control, PDEF over expression and PDEF TWIST overexpression

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: PC3

TEAD4 Antibody Western Blot validation (1:2500 dilution) in PC3 (Cat no:12418-1-AP)