• Featured Product
  • KD/KO Validated

TEAD4 Polyclonal antibody

TEAD4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12418-1-AP

Synonyms

EFTR 2, hRTEF 1B, RTEF1, TCF13L1, TEA domain family member 4, TEAD 4, TEAD4, TEF 3, TEF3, TEFR 1, Transcription factor 13 like 1, Transcription factor RTEF 1



Tested Applications

Positive WB detected inBGC-823 cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12418-1-AP targets TEAD4 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEAD4 fusion protein Ag3059
Full Name TEA domain family member 4
Calculated Molecular Weight 434 aa, 48 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC015497
Gene Symbol TEAD4
Gene ID (NCBI) 7004
RRIDAB_2203074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD4 belongs to a protein family that share the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins , a TBP-associated factor and a limiting transcriptional intermediary facotrs. TEAD4 also preforms an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but molecular weight of TEAD4 is detected about 50-55 kDa.

Protocols

Product Specific Protocols
WB protocol for TEAD4 antibody 12418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

Thyroid hormone regulates muscle fiber type conversion via miR-133a1.

Authors - Duo Zhang
humanWB

Cell Mol Biol Lett

KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway

Authors - Xiaomin Chen
humanCoIP

J Cell Mol Med

PMID: 31559693

Authors - Yangyang Yao
humanWB

Carcinogenesis

A yes-associated protein 1-Notch1 positive feedback loop promotes breast cancer lung metastasis by attenuating the Bone morphogenetic protein 4-SMAD family member 1/5 signaling

Authors - Lin Zhao
humanWB,CoIP

J Cell Mol Med

NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.

Authors - Bowen Hu
  • KD Validated
humanWB

Mol Carcinog

YAP/TEAD4-induced KIF4A contributes to the progression and worse prognosis of esophageal squamous cell carcinoma.

Authors - Ya Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Pramod Bahadur (Verified Customer) (03-05-2024)

We used this antibody for PC3 cell lines with vector control, PDEF over expression and PDEF TWIST overexpression

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: PC3
TEAD4 Antibody Western Blot validation (1:2500 dilution) in PC3 (Cat no:12418-1-AP)