Tested Applications
| Positive WB detected in | BGC-823 cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12418-1-AP targets TEAD4 in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3059 Product name: Recombinant human TEAD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 135-434 aa of BC015497 Sequence: SAQIISATAFHSSMALARGPGRPAVSGFWQGALPGQAGTSHDVKPFSQQTYAVQPPLPLPGFESPAGPAPSPSAPPAPPWQGRSVASSKLWMLEFSAFLEQQQDPDTYNKHLFVHIGQSSPSYSDPYLEAVDIRQIYDKFPEKKGGLKDLFERGPSNAFFLVKFWADLNTNIEDEGSSFYGVSSQYESPENMIITCSTKVCSFGKQVVEKVETEYARYENGHYSYRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTQETLLCIAYVFEVSASEHGAQHHIYRLVKE Predict reactive species |
| Full Name | TEA domain family member 4 |
| Calculated Molecular Weight | 434 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC015497 |
| Gene Symbol | TEAD4 |
| Gene ID (NCBI) | 7004 |
| RRID | AB_2203074 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15561 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TEAD4 belongs to a protein family that share the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins , a TBP-associated factor and a limiting transcriptional intermediary facotrs. TEAD4 also preforms an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but molecular weight of TEAD4 is detected about 50-55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TEAD4 antibody 12418-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-TEAD4 antibody (Cat# 12418-1-AP) has 47 citations spanning high-impact journals including Nature Metabolism, Nature Communications, ACS Nano, Advanced Science, Cell Reports, Journal of Biological Chemistry, and Journal of Experimental & Clinical Cancer Research. Research fields cover oncology (breast, colorectal, gastric, lung, liver, bladder, cervical, esophageal cancers), fibrosis, bone biology, pulmonary hypertension, ferroptosis, stem cell biology, and inflammatory diseases.
| Species | Application | Title |
|---|---|---|
Nat Metab Early-activated extracellular matrix proteins shape the metabolic and spatial dynamics of the kidney fibrotic microenvironment. | ||
Nat Commun Escherichia coli promotes colorectal cancer metastasis by maintaining enhancer-promoter loops through releasing neutrophil extracellular traps. | ||
ACS Nano Nano-Self-Assembled Particles Loaded with Aldehyde Dehydrogenase 2 Agonist Inhibit Ischemia-Reperfusion Injury of Transplanted Kidneys. | ||
J Exp Clin Cancer Res TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer.
| ||
Adv Sci (Weinh) TEAD1 Enhances Exosome Secretion and Promotes Exosome-Mediated Tissue Regeneration. |
Reviews
What customers say
The single review for this antibody is from a researcher who used it for Western Blot at a 1:2500 dilution on PC3 cell lines. They tested it across three conditions: vector control, PDEF overexpression, and PDEF TWIST overexpression. The reviewer gave the product a perfect 5-star rating, indicating strong performance and satisfaction with the antibody's results in their experimental setup.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Pramod Bahadur (Verified Customer) (03-05-2024) | We used this antibody for PC3 cell lines with vector control, PDEF over expression and PDEF TWIST overexpression
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