TFAM Monoclonal antibody

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  WB analysis using 68557-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68557-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 68557-5-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 68557-5-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68557-5-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (68557-5-Ig, Clone: 1C8H3 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

TFAM Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68557-5-Ig

Clone No.1C8H3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

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Tested Applications

• Positive WB detected in: Jurkat cells, Caco-2 cells, U2OS cells, LNCaP cells, MOLT-4 cells, MJ cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68557-5-Ig targets TFAM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFAM fusion protein Ag18253
• Full Name: transcription factor A, mitochondrial
• Calculated Molecular Weight: 246 aa, 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC126366
• Gene Symbol: TFAM
• Gene ID (NCBI): 7019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q00059
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.