TFAM Monoclonal antibody, PBS Only

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  WB analysis using 68557-5-Ig (same clone as 68557-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 68557-5-Ig (same clone as 68557-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (68557-5-Ig, Clone: 1C8H3 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

TFAM Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68557-5-PBS

Clone No.1C8H3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

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Product Information

68557-5-PBS targets TFAM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFAM fusion protein Ag18253
• Full Name: transcription factor A, mitochondrial
• Calculated Molecular Weight: 246 aa, 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC126366
• Gene Symbol: TFAM
• Gene ID (NCBI): 7019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q00059
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.