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  • KD/KO Validated

TIA1 Polyclonal antibody

TIA1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12133-2-AP

Synonyms

Nucleolysin TIA 1 isoform p40, p40 TIA 1, RNA binding protein TIA 1, TIA 1, TIA1



Tested Applications

Positive WB detected inCOLO 320 cells, mouse thymus tissue, mouse spleen tissue, Raji cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12133-2-AP targets TIA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIA1 fusion protein Ag2778
Full Name TIA1 cytotoxic granule-associated RNA binding protein
Calculated molecular weight 214 aa, 24 kDa, 43 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC015944
Gene symbol TIA1
Gene ID (NCBI) 7072
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43. Moreover, TDP-43 in SGs became less mobile and insoluble.

Protocols

Product Specific Protocols
WB protocol for TIA1 antibody 12133-2-APDownload protocol
IHC protocol for TIA1 antibody 12133-2-APDownload protocol
IF protocol for TIA1 antibody 12133-2-APDownload protocol
IP protocol for TIA1 antibody 12133-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Acta Neuropathol Commun

Clinical and neuropathological features of ALS/FTD with TIA1 mutations.

Authors - Veronica Hirsch-Reinshagen
humanWB

Int J Mol Sci

HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA.

Authors - Wenhui Zhao
  • KD Validated
ratIF

Int J Mol Med

miR‑335 promotes stress granule formation to inhibit apoptosis by targeting ROCK2 in acute ischemic stroke.

Authors - Wenwen Si
chickenWB

Vet Microbiol

Critical role for G3BP1 in infectious bursal disease virus (IBDV)-induced stress granule formation and viral replication.

Authors - Dianzheng Zhao
mouseWB

Stem Cells Int

Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells.

Authors - Zhe Geng
mouseWB

Methods

CLIP: construction of cDNA libraries for high-throughput sequencing from RNAs cross-linked to proteins in vivo.

Authors - Wang Zhen Z

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (12-28-2019)

PANC1 cells fixed in 4% paraformaldehyde and stained overnight at 4C. Bright staining, mix of nuclear and stress granule localization as expected.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human pancreatic adenocarcinoma
TIA1 Antibody Immunofluorescence, validation (1:500 dilution) in Human pancreatic adenocarcinoma (Cat no:12133-2-AP)
FH

Joshua (Verified Customer) (12-12-2018)

We found acetone fixation (20 min in 100% acetone at -20C) worked better than paraformaldehyde.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: IMR90 Human Lung Fibroblasts
TIA1 Antibody Immunofluorescence, validation (1:500 dilution) in IMR90 Human Lung Fibroblasts (Cat no:12133-2-AP)