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  • KD/KO Validated

TIA1 Polyclonal antibody

TIA1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 12133-2-AP


Nucleolysin TIA 1 isoform p40, p40 TIA 1, RNA binding protein TIA 1, TIA 1, TIA1

Tested Applications

Positive WB detected inCOLO 320 cells, Raji cells, mouse spleen tissue, mouse thymus tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells, HepG2 cells, HeLa cells, sodium arsenite treated HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12133-2-AP targets TIA1 in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIA1 fusion protein Ag2778
Full Name TIA1 cytotoxic granule-associated RNA binding protein
Calculated Molecular Weight 214 aa, 24 kDa, 43 kDa
Observed Molecular Weight ~40 kDa
GenBank Accession NumberBC015944
Gene Symbol TIA1
Gene ID (NCBI) 7072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43. Moreover, TDP-43 in SGs became less mobile and insoluble.


Product Specific Protocols
WB protocol for TIA1 antibody 12133-2-APDownload protocol
IHC protocol for TIA1 antibody 12133-2-APDownload protocol
IF protocol for TIA1 antibody 12133-2-APDownload protocol
IP protocol for TIA1 antibody 12133-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
  • KD Validated

Cell Res

Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures.

Authors - Lei Sun

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo


TIA1 Mutations in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia Promote Phase Separation and Alter Stress Granule Dynamics.

Authors - Ian R Mackenzie

Nat Commun

Cellular stress alters 3'UTR landscape through alternative polyadenylation and isoform-specific degradation.

Authors - Dinghai Zheng


Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia

Authors - Laura Pasetto


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


haibo (Verified Customer) (09-28-2020)

Western blotting was good. But can not see clear granule signal even after treatment by IF. The cells used were fibroblasts.

  • Applications: Western Blot, Immunofluorescence

Manohar (Verified Customer) (09-23-2020)

Used 1% Milk for blocking

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293

Joshua (Verified Customer) (12-28-2019)

PANC1 cells fixed in 4% paraformaldehyde and stained overnight at 4C. Bright staining, mix of nuclear and stress granule localization as expected.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human pancreatic adenocarcinoma
TIA1 Antibody Immunofluorescence, validation (1:500 dilution) in Human pancreatic adenocarcinoma (Cat no:12133-2-AP)

Joshua (Verified Customer) (12-12-2018)

We found acetone fixation (20 min in 100% acetone at -20C) worked better than paraformaldehyde.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: IMR90 Human Lung Fibroblasts
TIA1 Antibody Immunofluorescence, validation (1:500 dilution) in IMR90 Human Lung Fibroblasts (Cat no:12133-2-AP)