TIMP1 Polyclonal antibody

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  WB analysis using 32669-1-AP

  Rat lung tissue was subjected to SDS PAGE followed by western blot with 32669-1-AP (TIMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat lung using 32669-1-AP

  IP result of anti-TIMP1 (IP:32669-1-AP, 4ug; Detection:32669-1-AP 1:500) with rat lung tissue lysate 1200 ug.

TIMP1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 32669-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IP, ELISA

Synonyms

Metalloproteinase inhibitor 1, TIMP-1, Tissue inhibitor of metalloproteinases 1

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Tested Applications

• Positive WB detected in: rat lung tissue
• Positive IP detected in: rat lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32669-1-AP targets TIMP1 in WB, IP, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMP1 fusion protein Eg3212
• Full Name: TIMP metallopeptidase inhibitor 1
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: NM_053819.1
• Gene Symbol: Timp1
• Gene ID (NCBI): 116510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P30120
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. The glycosylation modification of TIMP-1 has a molecular weight of 28kDa (PMID: 9543631).