TIP47 Monoclonal antibody

TIP47 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4C11B1

Cat no : 66523-1-Ig

Synonyms

M6PRBP1, Perilipin 3, Mannose-6-phosphate receptor-binding protein 1, 4C11B1, 47 kDa MPR-binding protein

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Validation Data Gallery

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  WB analysis using 66523-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of U2OS using 66523-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 66523-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66523-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66523-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The F-actin was stained with CL594-Phalloidin (red).

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  IF Staining of HeLa using 66523-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, U2OS cells, A549 cells, A431 cells, LNCaP cells, K-562 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: oleic acid treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66523-1-Ig targets TIP47 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TIP47 fusion protein Ag1028
• Full Name: mannose-6-phosphate receptor binding protein 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC007566
• Gene Symbol: TIP47/PLIN3
• Gene ID (NCBI): 10226
• RRID: AB_2881886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Lipid-accumulated reactive astrocytes promote disease progression in epilepsy; Authors - Zhang-Peng Chen