TIRAP Polyclonal antibody

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  WB analysis using 26563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26563-1-AP (TIRAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26563-1-AP (TIRAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TIRAP Polyclonal Antibody for WB, ELISA

Cat No. 26563-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TIR domain-containing adapter protein, MyD88-2, MyD88 adapter-like protein, MyD88 adapter like protein, MAL

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Tested Applications

• Positive WB detected in: HT-29 cells, HCT 116 cells, HeLa cells, K-562 cells, SW480 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26563-1-AP targets TIRAP in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24230

  Product name: Recombinant human TIRAP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-235 aa of BC032474

  Sequence: MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCTIPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVKEAVMRYLQTLSWHLLYHGTPEIGVK

• Full Name: toll-interleukin 1 receptor (TIR) domain containing adaptor protein
• Calculated Molecular Weight: 256 aa, 28 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC032474
• Gene Symbol: TIRAP
• Gene ID (NCBI): 114609
• RRID: AB_3669548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P58753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIRAP, also known as MyD88-adaptor Like (MAL), is a crucial intracellular signaling molecule that plays a significant role in the regulation of immune responses, particularly in the context of Toll-like receptor (TLR)-mediated innate immune signaling. It functions as an adapter molecule that associates with receptor-mediated activation of host immune signaling. The innate immune system recognizes microbial pathogens through receptors, including TLRs, which identify pathogen-associated molecular patterns (PAMPs).