TMCO1 Polyclonal antibody, PBS Only

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  WB analysis using 27757-1-AP (same clone as 27757-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27757-1-AP (TMCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27757-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27757-1-AP (same clone as 27757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27757-1-AP (TMCO1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27757-1-PBS in a different storage buffer formulation.

TMCO1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27757-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

UNQ151/PRO177, TMCC4, RP11 466F5.7, PNAS 136, PCIA3

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Product Information

27757-1-PBS targets TMCO1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMCO1 fusion protein Ag26278
• Full Name: transmembrane and coiled-coil domains 1
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: NM_019026
• Gene Symbol: TMCO1
• Gene ID (NCBI): 54499
• RRID: AB_2880962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UM00
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TMCO1, also known as TMCC4, PNAS-10, and PNAS-136, is a 188 amino acid protein, which is Widely expressed in adult and fetal tissues, with higher levels in thymus, prostate, testis and small intestine and lower levels in brain, placenta, lung and kidney (PubMed:10393320, PubMed:20018682). TMCO1 as a Calcium-selective channel is required to prevent calcium stores from overfilling, thereby playing a key role in calcium homeostasis (PubMed:27212239). In response to endoplasmic reticulum overloading, TMCO1, assembles into a homotetramer, forming a functional calcium-selective channel, regulating the calcium content in endoplasmic reticulum store (PubMed:27212239).