|Positive IF detected in||human breast cancer tissue|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
CL488-60291 targets TNF Alpha in WB, IF applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||TNF Alpha fusion protein Ag11413|
|Full Name||tumor necrosis factor (TNF superfamily, member 2)|
|Calculated molecular weight||233 aa, 26 kDa|
|GenBank accession number||BC028148|
|Gene ID (NCBI)||7124|
|Conjugate||CoraLite® Plus 488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||493 nm / 522 nm|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
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