TNF Alpha Polyclonal antibody

TNF Alpha Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 26405-1-AP

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor



Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26405-1-AP is TNF Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNF Alpha fusion protein Ag24020
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC028148
Gene symbol TNF
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

Protocols

Product Specific Protocols
WB protocol for TNF Alpha antibody 26405-1-APDownload protocol
IHC protocol for TNF Alpha antibody 26405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols