TNF-alpha Polyclonal antibody, PBS Only

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  WB analysis using 26405-1-AP (same clone as 26405-1-PBS)

  PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 26405-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody 60004-1-Ig as loading control. This data was developed using the same antibody clone with 26405-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 26405-1-AP (same clone as 26405-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26405-1-AP (TNF Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26405-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 26405-1-AP (same clone as 26405-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26405-1-AP (TNF Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26405-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 26405-1-AP (same clone as 26405-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26405-1-AP (TNF Alpha antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26405-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 26405-1-AP (same clone as 26405-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using TNF-alpha antibody (26405-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26405-1-PBS in a different storage buffer formulation.

TNF-alpha Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26405-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TNF, TNF alpha, TNF-a, C-domain 1, C-domain 2

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Product Information

26405-1-PBS targets TNF-alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNF-alpha fusion protein Ag24020
• Full Name: tumor necrosis factor (TNF superfamily, member 2)
• Calculated Molecular Weight: 233 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC028148
• Gene Symbol: TNF-alpha
• Gene ID (NCBI): 7124
• ENSEMBL Gene ID: ENSG00000232810
• RRID: AB_2918102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01375
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.