TNP1 Polyclonal antibody

TNP1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 17178-1-AP

Synonyms

STP 1, TNP1, TP 1, TP1



Tested Applications

Positive WB detected inmouse testis tissue
Positive IF detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17178-1-AP targets TNP1 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNP1 fusion protein Ag10890
Full Name transition protein 1 (during histone to protamine replacement)
Calculated molecular weight 55 aa, 7 kDa
Observed molecular weight 6-10 kDa
GenBank accession numberBC029516
Gene symbol TNP1
Gene ID (NCBI) 7141
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.

Protocols

Product Specific Protocols
WB protocol for TNP1 antibody 17178-1-APDownload protocol
IF protocol for TNP1 antibody 17178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis.

Authors - Chang Rok Kim
mouseIF

Zygote

CFAP43-mediated intra-manchette transport is required for sperm head shaping and flagella formation.

Authors - Yi Yu
mouseIHC

Reprod Sci

Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.

Authors - Esakky Prabagaran P
mouseIF

Dev Biol

The ubiquitin ligase subunit β-TrCP in Sertoli cells is essential for spermatogenesis in mice.

Authors - Akane Morohoshi
mouseIF

Biol Reprod

Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in mice†.

Authors - Darius J Devlin
mouseWB

J Cell Sci

TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis.

Authors - Kula N Jha

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chongil (Verified Customer) (08-19-2021)

This is a great IF antibody to detect Tnp1 protein.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: Mouse testis section
TNP1 Antibody Immunofluorescence validation (1:800 dilution) in Mouse testis section (Cat no:17178-1-AP)
FH

Zhenminm (Verified Customer) (07-08-2019)

This antibody is highly specific and works excellently for immunohistochemistry as well as immunofluorescence in formalin fixed and paraffin embedded tissue sections.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Mouse Testis
TNP1 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1,000 dilution) in Mouse Testis (Cat no:17178-1-AP)