|Positive WB detected in||mouse testis tissue|
|Positive IF detected in||mouse testis tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17178-1-AP targets TNP1 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TNP1 fusion protein Ag10890|
|Full Name||transition protein 1 (during histone to protamine replacement)|
|Calculated molecular weight||55 aa, 7 kDa|
|Observed molecular weight||6-10 kDa|
|GenBank accession number||BC029516|
|Gene ID (NCBI)||7141|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.
PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis.
CFAP43-mediated intra-manchette transport is required for sperm head shaping and flagella formation.
Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.
The ubiquitin ligase subunit β-TrCP in Sertoli cells is essential for spermatogenesis in mice.
Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in mice†.
J Cell Sci
TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chongil (Verified Customer) (08-19-2021)
This is a great IF antibody to detect Tnp1 protein.
Zhenminm (Verified Customer) (07-08-2019)
This antibody is highly specific and works excellently for immunohistochemistry as well as immunofluorescence in formalin fixed and paraffin embedded tissue sections.