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  • KD/KO Validated

TPPII Polyclonal antibody

TPPII Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14120-1-AP

Synonyms

TPP 2, TPP II, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, Tripeptidyl peptidase II



Tested Applications

Positive WB detected inJurkat cells, Jurkat
Positive IP detected inJurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14120-1-AP targets TPPII in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPPII fusion protein Ag5296
Full Name tripeptidyl peptidase II
Calculated molecular weight 1249 aa, 138 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC039905
Gene symbol TPP2
Gene ID (NCBI) 7174
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

Protocols

Product Specific Protocols
WB protocol for TPPII antibody 14120-1-APDownload protocol
IHC protocol for TPPII antibody 14120-1-APDownload protocol
IF protocol for TPPII antibody 14120-1-APDownload protocol
IP protocol for TPPII antibody 14120-1-APDownload protocol
FC protocol for TPPII antibody 14120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

The Activation State of CD4 T Cells Alters Cellular Peptidase Activities, HIV Antigen Processing, and MHC Class I Presentation in a Sequence-Dependent Manner.

Authors - Julie Boucau
humanWB,IF

Genes Cancer

Tripeptidyl Peptidase II Is Required for c-MYC-Induced Centriole Overduplication and a Novel Therapeutic Target in c-MYC-Associated Neoplasms.

Authors - Stefan Duensing
  • KD Validated
humanIF

Mol Cell Biochem

Novel protein-protein interactions of TPPII, p53, and SIRT7.

Authors - Jarmila Nahálková
humanWB

J Immunol

Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen.

Authors - Nathalie Vigneron