TPT1 Monoclonal antibody

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  WB analysis of MCF-7 using 66713-1-Ig

  WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells.

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  WB analysis using 66713-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66713-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66713-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66713-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66713-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 66713-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HEK-293 using 66713-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

TPT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66713-1-Ig

Clone No.1G6A10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TCTP, p23, HRF, Histamine-releasing factor, Fortilin

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, RAW 264.7 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66713-1-Ig targets TPT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27651

  Product name: Recombinant human TPT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-172 aa of BC003352

  Sequence: MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC

• Full Name: tumor protein, translationally-controlled 1
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003352
• Gene Symbol: TPT1
• Gene ID (NCBI): 7178
• RRID: AB_2882064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P13693
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.