TPX2 Polyclonal antibody, PBS Only

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  WB analysis using 11741-1-AP (same clone as 11741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11741-1-AP (same clone as 11741-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

  IP result of anti-TPX2 (IP:11741-1-AP, 4ug; Detection:11741-1-AP 1:800) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11741-1-AP (same clone as 11741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11741-1-AP (TPX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11741-1-AP (same clone as 11741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11741-1-AP (same clone as 11741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11741-1-AP (same clone as 11741-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (11741-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11741-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

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Product Information

11741-1-PBS targets TPX2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPX2 fusion protein Ag2334
• Full Name: TPX2, microtubule-associated, homolog (Xenopus laevis)
• Calculated Molecular Weight: 747 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC020207
• Gene Symbol: TPX2
• Gene ID (NCBI): 22974
• RRID: AB_2208895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULW0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).