|Positive WB detected in||HepG2 cells, mouse liver tissue, mouse spleen tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15753-1-AP targets TRBP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TRBP fusion protein Ag8255|
|Full Name||TAR (HIV-1) RNA binding protein 2|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||43-46 kDa|
|GenBank accession number||BC005860|
|Gene ID (NCBI)||6895|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43–46 kDa, are expressed in human cells. (PMID: 16142218)
|Product Specific Protocols|
|WB protocol for TRBP antibody 15753-1-AP||Download protocol|
|IHC protocol for TRBP antibody 15753-1-AP||Download protocol|
|IF protocol for TRBP antibody 15753-1-AP||Download protocol|
|IP protocol for TRBP antibody 15753-1-AP||Download protocol|
|Click here to view our Standard Protocols|
FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.
Mol Biol Cell
A Biosensor for MAPK-Dependent Lin28 Signaling.
Biochem Biophys Res Commun
The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection.
Dietary daidzein inhibits hepatitis C virus replication by decreasing microRNA-122 levels.
J Mol Biol
Evidence of A Negative Feedback Network Between TDP-43 and miRNAs Dependent on TDP-43 Nuclear Localization.
Nucleic Acids Res
S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.