Tested Applications
| Positive WB detected in | HepG2 cells, mouse liver tissue, mouse spleen tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15753-1-AP targets TRBP in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8255 Product name: Recombinant human TRBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC005860 Sequence: MSEEEQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGDTSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAATPVPSVVLTRSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGEAARRALQYLKIMAGSK Predict reactive species |
| Full Name | TAR (HIV-1) RNA binding protein 2 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 43-46 kDa |
| GenBank Accession Number | BC005860 |
| Gene Symbol | TRBP |
| Gene ID (NCBI) | 6895 |
| RRID | AB_2200485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRBP antibody 15753-1-AP | Download protocol |
| IHC protocol for TRBP antibody 15753-1-AP | Download protocol |
| IP protocol for TRBP antibody 15753-1-AP | Download protocol |
| WB protocol for TRBP antibody 15753-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRBP polyclonal antibody (Cat# 15753-1-AP) has 26 citations across journals including Nature, Nature Communications, Molecular Cell, Nucleic Acids Research, Cell Death & Disease, Acta Neuropathologica, and Oncogene. Research fields span RNA biology (miRNA processing, RISC complex), oncology (gastric, liver, breast, ovarian, and cervical cancers), neurology (ALS, Fragile X, Alzheimer's), virology (HIV, HCV, CMV), and immunology.
| Species | Application | Title |
|---|---|---|
Nature FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing. | ||
Nat Commun Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells. | ||
Mol Cell Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.
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Reviews
What customers say
The product has one verified review from a user who tested it for Western Blot and Immunoprecipitation using lot 00047911. The reviewer rated it 5 out of 5 stars and described the performance as "GOOD." Overall, the limited feedback is positive, indicating satisfactory results in both WB and IP applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
vijayalakshmi (Verified Customer) (02-05-2026) | GOOD
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