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TRIM24 Polyclonal antibody
TRIM24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 14208-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, L02 cells, BGC-823 cells, human heart tissue, mouse skeletal muscle tissue, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
14208-1-AP targets TRIM24 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TRIM24 fusion protein Ag5417 |
Full Name | tripartite motif-containing 24 |
Calculated Molecular Weight | 117 kDa |
Observed Molecular Weight | 117 kDa |
GenBank Accession Number | BC028689 |
Gene Symbol | TRIM24 |
Gene ID (NCBI) | 8805 |
RRID | AB_2256646 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TRIM24 antibody 14208-1-AP | Download protocol |
IHC protocol for TRIM24 antibody 14208-1-AP | Download protocol |
IF protocol for TRIM24 antibody 14208-1-AP | Download protocol |
IP protocol for TRIM24 antibody 14208-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression
| ||
Mol Cell TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains. | ||
Nat Chem Biol Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Parijat (Verified Customer) (09-20-2023) | Good antibody for Western
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