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  • KD/KO Validated

TRIM24 Polyclonal antibody

TRIM24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 14208-1-AP

Synonyms

TIF1-alpha, TIF1A, TIF1, RING-type E3 ubiquitin transferase TIF1-alpha, EC:2.3.2.27



Tested Applications

Positive WB detected inHeLa cells, L02 cells, BGC-823 cells, human heart tissue, mouse skeletal muscle tissue, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14208-1-AP targets TRIM24 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM24 fusion protein Ag5417
Full Name tripartite motif-containing 24
Calculated Molecular Weight 117 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC028689
Gene Symbol TRIM24
Gene ID (NCBI) 8805
RRIDAB_2256646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

Protocols

Product Specific Protocols
WB protocol for TRIM24 antibody 14208-1-APDownload protocol
IHC protocol for TRIM24 antibody 14208-1-APDownload protocol
IF protocol for TRIM24 antibody 14208-1-APDownload protocol
IP protocol for TRIM24 antibody 14208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nature

TRIM24 links a non-canonical histone signature to breast cancer.

Authors - Tsai Wen-Wei WW
humanWB,CoIP

Mol Cell

p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression

Authors - Monisankar Ghosh
  • KD Validated
humanWB

Mol Cell

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.

Authors - Ai Kaiho-Soma
humanWB

Nat Chem Biol

Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands.

Authors - Lara N Gechijian
humanWB, IP, IHC

Nat Commun

TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma.

Authors - Deguan Lv
humanWB,IF

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Parijat (Verified Customer) (09-20-2023)

Good antibody for Western

  • Applications: Western Blot