|Positive WB detected in||HeLa cells, L02 cells, human heart tissue, mouse skeletal muscle tissue, BGC-823 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14208-1-AP targets TRIM24 in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TRIM24 fusion protein Ag5417|
|Full Name||tripartite motif-containing 24|
|Calculated molecular weight||117 kDa|
|Observed molecular weight||117 kDa|
|GenBank accession number||BC028689|
|Gene ID (NCBI)||8805|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.
|Product Specific Protocols|
|WB protocol for TRIM24 antibody 14208-1-AP||Download protocol|
|IHC protocol for TRIM24 antibody 14208-1-AP||Download protocol|
|IF protocol for TRIM24 antibody 14208-1-AP||Download protocol|
|IP protocol for TRIM24 antibody 14208-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis.
Biomed Res Int
Overexpression of TRIM24 Stimulates Proliferation and Glucose Metabolism of Head and Neck Squamous Cell Carcinoma.
Clinical significance and biological roles of TRIM24 in human bladder carcinoma.
Cell Biol Int
E3 ubiquitin ligase TRIM24 deficiency promotes NLRP3/caspase-1/IL-1β-mediated pyroptosis in endometriosis.
TRIM24 is upregulated in human gastric cancer and promotes gastric cancer cell growth and chemoresistance.
TRIM24 as an independent prognostic biomarker for prostate cancer.