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  • KD/KO Validated

TRIM24 Polyclonal antibody

TRIM24 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP,ELISA

Conjugate

Unconjugated

Cat no : 14208-1-AP

Synonyms

hTIF1, PTC6, RING finger protein 82, RNF82, TF1A, TIF1, TIF1 alpha, TIF1A, TIF1ALPHA, TRIM24, tripartite motif containing 24



Tested Applications

Positive WB detected inHeLa cells, L02 cells, human heart tissue, mouse skeletal muscle tissue, BGC-823 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14208-1-AP targets TRIM24 in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM24 fusion protein Ag5417
Full Name tripartite motif-containing 24
Calculated molecular weight 117 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC028689
Gene symbol TRIM24
Gene ID (NCBI) 8805
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

Protocols

Product Specific Protocols
WB protocol for TRIM24 antibody 14208-1-APDownload protocol
IHC protocol for TRIM24 antibody 14208-1-APDownload protocol
IF protocol for TRIM24 antibody 14208-1-APDownload protocol
IP protocol for TRIM24 antibody 14208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis.

Authors - Juan F Correa-Vázquez
humanWB,IHC

Biomed Res Int

Overexpression of TRIM24 Stimulates Proliferation and Glucose Metabolism of Head and Neck Squamous Cell Carcinoma.

Authors - Hongming Wang
  • KD Validated
IHC

Tumour Biol

Clinical significance and biological roles of TRIM24 in human bladder carcinoma.

Authors - Dongwei Xue
humanIHC

Cell Biol Int

E3 ubiquitin ligase TRIM24 deficiency promotes NLRP3/caspase-1/IL-1β-mediated pyroptosis in endometriosis.

Authors - Yuanyuan Hang
humanWB, IHC

Virchows Arch

TRIM24 is upregulated in human gastric cancer and promotes gastric cancer cell growth and chemoresistance.

Authors - Zhi-Feng Miao
humanIHC

Urol Oncol

TRIM24 as an independent prognostic biomarker for prostate cancer.

Authors - Anne Offermann