Validation Data Gallery
|Positive WB detected in||HeLa cells, L02 cells, BGC-823 cells, human heart tissue, mouse skeletal muscle tissue, MCF-7 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14208-1-AP targets TRIM24 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TRIM24 fusion protein Ag5417|
|Full Name||tripartite motif-containing 24|
|Calculated molecular weight||117 kDa|
|Observed molecular weight||117 kDa|
|GenBank accession number||BC028689|
|Gene ID (NCBI)||8805|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.
|Product Specific Protocols|
|WB protocol for TRIM24 antibody 14208-1-AP||Download protocol|
|IHC protocol for TRIM24 antibody 14208-1-AP||Download protocol|
|IF protocol for TRIM24 antibody 14208-1-AP||Download protocol|
|IP protocol for TRIM24 antibody 14208-1-AP||Download protocol|
|Click here to view our Standard Protocols|
TRIM24 links a non-canonical histone signature to breast cancer.
p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression
TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.
Nat Chem Biol
Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands.
TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma.
Identification of proximal SUMO-dependent interactors using SUMO-ID.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Parijat (Verified Customer) (09-20-2023)
Good antibody for Western