Validation Data Gallery
|Positive WB detected in||HeLa cells, rat spleen tissue, mouse testis tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15202-1-AP targets KAP1 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KAP1 fusion protein Ag7350|
|Full Name||tripartite motif-containing 28|
|Calculated molecular weight||89 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC004978|
|Gene ID (NCBI)||10155|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )
|Product Specific Protocols|
|WB protocol for KAP1 antibody 15202-1-AP||Download protocol|
|IHC protocol for KAP1 antibody 15202-1-AP||Download protocol|
|IF protocol for KAP1 antibody 15202-1-AP||Download protocol|
|IP protocol for KAP1 antibody 15202-1-AP||Download protocol|
|Click here to view our Standard Protocols|
TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.
DNA Repair (Amst)
ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress.
TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.
TRIM28 maintains genome imprints and regulates development of porcine SCNT embryos.
WASH interacts with Ku to regulate DNA double-stranded break repair.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Isabelle Cristine (Verified Customer) (01-14-2022)
This antibody works extremely well and is very specific for detecting the KAP1 protein in U2OS cells.