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  • KD/KO Validated

KAP1 Polyclonal antibody

KAP1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 15202-1-AP


KAP 1, KAP1, KRAB associated protein 1, KRAB interacting protein 1, KRIP 1, Nuclear corepressor KAP 1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1B, TRIM28, tripartite motif containing 28

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15202-1-AP targets KAP1 in WB, IP, IF, FC, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KAP1 fusion protein Ag7350
Full Name tripartite motif-containing 28
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC004978
Gene Symbol TRIM28
Gene ID (NCBI) 10155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )


Product Specific Protocols
WB protocol for KAP1 antibody 15202-1-APDownload protocol
IHC protocol for KAP1 antibody 15202-1-APDownload protocol
IF protocol for KAP1 antibody 15202-1-APDownload protocol
IP protocol for KAP1 antibody 15202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

Authors - Zhiwen Ding
mouseWB, IHC


Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Authors - Bojkowska Karolina K
  • KO Validated

J Exp Med

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

Authors - Longfei Ma

Nucleic Acids Res

Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency.

Authors - Weihua Qin
  • KO Validated

Proc Natl Acad Sci U S A

TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks.

Authors - Min Li

Cell Rep

POGZ suppresses 2C transcriptional program and retrotransposable elements

Authors - Xiaoyun Sun


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Katherine (Verified Customer) (09-25-2023)

Nice quality!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T

Henar (Verified Customer) (09-03-2022)

Great antibody very specific, I will use always

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000

Isabelle Cristine (Verified Customer) (01-14-2022)

This antibody works extremely well and is very specific for detecting the KAP1 protein in U2OS cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS
KAP1 Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:15202-1-AP)