TRIM33 Recombinant antibody, PBS Only

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  WB analysis using 85364-3-RR (same clone as 85364-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 85364-3-RR (same clone as 85364-3-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85364-3-RR (same clone as 85364-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM33 antibody (85364-3-RR, Clone: 242728H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

TRIM33 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85364-3-PBS

Clone No.242728H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Protein Rfg7, KIAA1113, Ectodermin homolog, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM33

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Product Information

85364-3-PBS targets TRIM33 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: tripartite motif-containing 33
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_015906
• Gene Symbol: TRIM33
• Gene ID (NCBI): 51592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UPN9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRIM33, also named KIAA1113, RFG7, and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion, and degradation via the ubiquitin-proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight detected is about 140-150 kDa the same as the result from the paper(PMID:25639486;26592194).