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  • Western Blot (WB) analysis of various lysates using TRIM33 Recombinant antibody (85364-3-RR)

    WB analysis using 85364-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TRIM33 Recombinant antibody (85364-3-RR)

    WB analysis of COLO 320 using 85364-3-RR

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIM33 Recombinant antibody (85364-3-RR)

    IF Staining of HepG2 using 85364-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM33 antibody (85364-3-RR, Clone: 242728H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TRIM33 Recombinant antibody

TRIM33 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85364-3-RR
Clone No.242728H2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Protein Rfg7, KIAA1113, Ectodermin homolog, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM33

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inMCF-7 cells, COLO 320 cells, MOLT-4 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85364-3-RR targets TRIM33 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name tripartite motif-containing 33
Calculated Molecular Weight 122 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberNM_015906
Gene Symbol TRIM33
Gene ID (NCBI) 51592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UPN9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM33, also named KIAA1113, RFG7, and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion, and degradation via the ubiquitin-proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight detected is about 140-150 kDa the same as the result from the paper(PMID:25639486;26592194).

Protocols

Product Specific Protocols
WB protocol for TRIM33 antibody 85364-3-RRDownload protocol
IF protocol for TRIM33 antibody 85364-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols