TRIM58 Polyclonal antibody

• 

  WB analysis using 28725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28725-1-AP (TRIM58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TRIM58 Polyclonal Antibody for WB, ELISA

Cat No. 28725-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

tripartite motif containing 58, TRIM, Protein BIA2, BIA2

---

Tested Applications

• Positive WB detected in: PC-3 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28725-1-AP targets TRIM58 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM58 fusion protein Ag30014
• Full Name: tripartite motif-containing 58
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_015431
• Gene Symbol: TRIM58
• Gene ID (NCBI): 25893
• RRID: AB_3669668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NG06
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM58 (Tripartite motif-containing protein 58) is an E3 ubiquitin ligase induced during late erythropoiesis. It can directly bind and ubiquitinate the intermediate chain of the microtubule motor dynein (DYNC1LI1/DYNC1LI2), stimulating the degradation of the dynein holoprotein complex. May participate in the erythroblast enucleation process through regulation of nuclear polarization (PMID: 25241935).