TRPV1 Monoclonal antibody

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  WB analysis of HepG2 using 66983-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-468 using 66983-1-Ig

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 66983-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66983-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 66983-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66983-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TRPV1 Monoclonal Antibody for WB, ELISA

Cat No. 66983-1-Ig

Clone No.1A3C9

Publications(48)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, CoIP, ELISA

Synonyms

Capsaicin receptor, DKFZp434K0220, Osm 9 like TRP channel 1, OTRPC1, TRPV1, Vanilloid receptor 1, VR1

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Tested Applications

• Positive WB detected in: HepG2 cells, MDA-MB-468 cells, T-47D cells, HEK-293 cells, Y79 cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 36 publications below
• CoIP: See 1 publications below

Product Information

66983-1-Ig targets TRPV1 in WB, CoIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, sheep
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRPV1 fusion protein Ag18683
• Full Name: transient receptor potential cation channel, subfamily V, member 1
• Calculated Molecular Weight: 839 aa, 95 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC132820
• Gene Symbol: TRPV1
• Gene ID (NCBI): 7442
• RRID: AB_2882302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NER1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).

Publications

Species	Application	Title
	WB	Phytomedicine; Yu-Xue-Bi capsule ameliorates aggressive synovitis and joint damage in rheumatoid arthritis via modulating the SUCNR1/HIF-1α/TRPV1 axis; Authors - Wenjia Chen
		Int J Mol Sci; The Contribution of TSLP Activation to Hyperalgesia in Dorsal Root Ganglia Neurons of a Rat.; Authors - Chun-Ching Lu
	WB	Biochim Biophys Acta Mol Basis Dis; Palmitic acid and trans-4-hydroxy-3-methoxycinnamate, the active ingredients of Yaobishu formula, reduce inflammation and pain by regulating gut microbiota and metabolic changes after lumbar disc herniation to activate autophagy and the Wnt/β-catenin pathway; Authors - ShuoQi Li
human	WB	EBioMedicine; Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis; Authors - Zhongyang Lv
human	WB	Int J Mol Sci; Luteolin Alleviates Oxidative Stress in Chronic Obstructive Pulmonary Disease Induced by Cigarette Smoke via Modulation of the TRPV1 and CYP2A13/NRF2 Signaling Pathways; Authors - Lina Zhou
human	WB	Front Mol Biosci; Role of TRPV1 ion channel in cervical squamous cell carcinoma genesis; Authors - Zhenming Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shihab (Verified Customer) (09-17-2021)

This antibody was tried in a cell type (cannot disclose) which has previously been shown to express plenty of TRPV1 signal. However, this antibody was unable to give any signal in various attempts at staining, even at 1:100 dilution. This is not to say that this anybody is not good because I have had trouble with various TRPV1 antibodies in this tissue type but it will be used in other cell/tissue type to see its staining capabilities

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100