TRPV1 Monoclonal antibody

TRPV1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

16

CloneNo.

1A3C9

Reviews (1)

Cat no : 66983-1-Ig

Synonyms

Capsaicin receptor, DKFZp434K0220, Osm 9 like TRP channel 1, OTRPC1, TRPV1, Vanilloid receptor 1, VR1

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Validation Data Gallery

• 

  WB analysis of HepG2 using 66983-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-468 using 66983-1-Ig

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66983-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66983-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 66983-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66983-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, MDA-MB-468 cells, T-47D cells, HEK-293 cells, Y79 cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

66983-1-Ig targets TRPV1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, sheep
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRPV1 fusion protein Ag18683
• Full Name: transient receptor potential cation channel, subfamily V, member 1
• Calculated molecular weight: 839 aa, 95 kDa
• Observed molecular weight: 105 kDa
• GenBank accession number: BC132820
• Gene symbol: TRPV1
• Gene ID (NCBI): 7442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).

Publications

Species	Application	Title
rat	IF	Int J Mol Sci; The Contribution of TSLP Activation to Hyperalgesia in Dorsal Root Ganglia Neurons of a Rat.; Authors - Chun-Ching Lu
human,mouse	WB,IF	EBioMedicine; Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis; Authors - Zhongyang Lv
human	WB	J Inflamm Res; Cannabidiol Inhibits Inflammation Induced by Cutibacterium acnes-Derived Extracellular Vesicles via Activation of CB2 Receptor in Keratinocytes; Authors - Ziqi Jiang
human,mouse	WB	Cell Calcium; Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury.; Authors - Zhenhong Liu
rat	WB,IHC	Front Pharmacol; Effect of Xiongmatang Extract on Behavioral and TRPV1-CGRP/CGRP-R Pathway in Rats With Migraine.; Authors - Dingding Liu
human	WB	Mol Hum Reprod; TRPV2, a novel player in the human ovary and human granulosa cells; Authors - Katja Eubler

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shihab (Verified Customer) (09-17-2021)

This antibody was tried in a cell type (cannot disclose) which has previously been shown to express plenty of TRPV1 signal. However, this antibody was unable to give any signal in various attempts at staining, even at 1:100 dilution. This is not to say that this anybody is not good because I have had trouble with various TRPV1 antibodies in this tissue type but it will be used in other cell/tissue type to see its staining capabilities

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100