TRPV1 Monoclonal antibody

TRPV1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C9

Cat no : 66983-1-Ig

Synonyms

Capsaicin receptor, DKFZp434K0220, Osm 9 like TRP channel 1, OTRPC1, TRPV1, Vanilloid receptor 1, VR1



Tested Applications

Positive WB detected inHepG2 cells, MDA-MB-468 cells, T-47D cells, HEK-293 cells, Y79 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

66983-1-Ig targets TRPV1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, sheep
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRPV1 fusion protein Ag18683
Full Name transient receptor potential cation channel, subfamily V, member 1
Calculated molecular weight 839 aa, 95 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC132820
Gene symbol TRPV1
Gene ID (NCBI) 7442
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).

Protocols

Product Specific Protocols
WB protocol for TRPV1 antibody 66983-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Int J Mol Sci

The Contribution of TSLP Activation to Hyperalgesia in Dorsal Root Ganglia Neurons of a Rat.

Authors - Chun-Ching Lu
ratWB

Biomed Eng Online

Effect of local ozone treatment on rats with anterior rectal resection and the possible mechanisms.

Authors - Wei Zhang
sheepIHC

eNeuro

Functional Characterization of Ovine Dorsal Root Ganglion Neurons Reveal Peripheral Sensitization after Osteochondral Defect.

Authors - Sampurna Chakrabarti
ratWB,IHC

Front Pharmacol

Effect of Xiongmatang Extract on Behavioral and TRPV1-CGRP/CGRP-R Pathway in Rats With Migraine.

Authors - Dingding Liu
human,mouseWB

Cell Calcium

Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury.

Authors - Zhenhong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shihab (Verified Customer) (09-17-2021)

This antibody was tried in a cell type (cannot disclose) which has previously been shown to express plenty of TRPV1 signal. However, this antibody was unable to give any signal in various attempts at staining, even at 1:100 dilution. This is not to say that this anybody is not good because I have had trouble with various TRPV1 antibodies in this tissue type but it will be used in other cell/tissue type to see its staining capabilities

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100