|Positive WB detected in||HepG2 cells, MDA-MB-468 cells, T-47D cells, HEK-293 cells, Y79 cells, HUVEC cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
66983-1-Ig targets TRPV1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, rat, sheep|
|Host / Isotype||Mouse / IgG1|
|Immunogen||TRPV1 fusion protein Ag18683|
|Full Name||transient receptor potential cation channel, subfamily V, member 1|
|Calculated molecular weight||839 aa, 95 kDa|
|Observed molecular weight||105 kDa|
|GenBank accession number||BC132820|
|Gene ID (NCBI)||7442|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).
Int J Mol Sci
The Contribution of TSLP Activation to Hyperalgesia in Dorsal Root Ganglia Neurons of a Rat.
Biomed Eng Online
Effect of local ozone treatment on rats with anterior rectal resection and the possible mechanisms.
Functional Characterization of Ovine Dorsal Root Ganglion Neurons Reveal Peripheral Sensitization after Osteochondral Defect.
Effect of Xiongmatang Extract on Behavioral and TRPV1-CGRP/CGRP-R Pathway in Rats With Migraine.
Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Shihab (Verified Customer) (09-17-2021)
This antibody was tried in a cell type (cannot disclose) which has previously been shown to express plenty of TRPV1 signal. However, this antibody was unable to give any signal in various attempts at staining, even at 1:100 dilution. This is not to say that this anybody is not good because I have had trouble with various TRPV1 antibodies in this tissue type but it will be used in other cell/tissue type to see its staining capabilities