TSG101 Monoclonal antibody

TSG101 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7G8

Cat no : 67381-1-Ig

Synonyms

Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker, TSG10, TSG101, tumor susceptibility gene 101, VPS23



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HSC-T6 cells, Neuro-2a cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, human colon cancer tissue
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 8 publications below

Product Information

67381-1-Ig targets TSG101 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TSG101 fusion protein Ag28569
Full Name tumor susceptibility gene 101
Calculated molecular weight 44 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC002487
Gene symbol TSG101
Gene ID (NCBI) 7251
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Protocols

Product Specific Protocols
WB protocol for TSG101 antibody 67381-1-IgDownload protocol
IHC protocol for TSG101 antibody 67381-1-IgDownload protocol
IF protocol for TSG101 antibody 67381-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway.

Authors - Shaohua Wang
mouseWB

Oxid Med Cell Longev

Adipose-Derived Circulating Exosomes Promote Protection of the Pulmonary Endothelial Barrier by Inhibiting EndMT and Oxidative Stress through Down-Regulation of the TGF-β Pathway: A Potential Explanation for the Obesity Paradox in ARDS.

Authors - Di Qi
humanWB

Front Cardiovasc Med

Circulating Exosomal miR-181b-5p Promoted Cell Senescence and Inhibited Angiogenesis to Impair Diabetic Foot Ulcer via the Nuclear Factor Erythroid 2-Related Factor 2/Heme Oxygenase-1 Pathway.

Authors - Shaohua Wang
humanWB

Front Pharmacol

Engineered Exosomes-Mediated Transfer of hsa-miR-320a Overcomes Chemoresistance in Cervical Cancer Cells via Targeting MCL1.

Authors - Jinling Zhou
humanWB

Front Microbiol

Proteomics Analysis of Exosomes From Patients With Active Tuberculosis Reveals Infection Profiles and Potential Biomarkers.

Authors - Min Zhang
WB

Int J Biol Macromol

Exosomal miRNA-181a-5p from the cells of the hair follicle dermal papilla promotes the hair follicle growth and development via the Wnt/β-catenin signaling pathway.

Authors - Bohao Zhao