CoraLite® Plus 488-conjugated TSG101 Monoclonal antibody

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  IF Staining of human colon cancer using CL488-67381

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL488-67381

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

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  FC experiment of THP-1 using CL488-67381

  1X10^6 THP-1 cells were stained with 0.2 ug CoraLite® Plus 488 Anti-Human TSG101 (CL488-67381, Clone: 2B7G8) (red), or 0.2 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TSG101 Monoclonal Antibody for IF-P, FC (Intra)

Cat No. CL488-67381

Clone No.2B7G8

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

IF-P, FC (Intra)

Synonyms

VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue
• Positive FC (Intra) detected in: THP-1 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

CL488-67381 targets TSG101 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: goat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TSG101 fusion protein Ag28569
• Full Name: tumor susceptibility gene 101
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC002487
• Gene Symbol: TSG101
• Gene ID (NCBI): 7251
• RRID: AB_2919462
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99816
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Publications

Species	Application	Title
goat	FC	Comp Biochem Physiol Part D Genomics Proteomics; Exosomes serve as a crucial mediator of epithelial-fibroblast communication during hair follicle morphogenesis in cashmere goats; Authors - Erhan Hai