TSHR Polyclonal antibody

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  WB analysis of HeLa using 14450-1-AP

  WB result of TSHR antibody (14450-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSHR transfected HeLa cells.

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  WB analysis using 14450-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14450-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14450-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 14450-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14450-1-AP (TSHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TSHR Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14450-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

TSH R, Thyrotropin receptor, Thyroid-stimulating hormone receptor, LGR3, hTSHR I

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Tested Applications

• Positive WB detected in: TT cells, HeLa cells, mouse ovary tissue, mouse thymus tissue
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

14450-1-AP targets TSHR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5957

  Product name: Recombinant human TSHR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-253 aa of BC063613

  Sequence: MRPADLLQLVLLLDLPRDLGGMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLLPLGRKSLSFETQKAPRSSMPS

• Full Name: thyroid stimulating hormone receptor
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 87kDa, 62-70kDa, 28-30kDa
• GenBank Accession Number: BC063613
• Gene Symbol: TSHR
• Gene ID (NCBI): 7253
• RRID: AB_2208263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16473
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein hormone produced by thyrotrophs in the anterior pituitary gland. The TSH receptor (TSHR), mainly expressed on the thyroid epithelial cells, is a G protein-coupled receptor (GPCR) of the seven-transmembrane domain family and plays a central role in controlling thyroid cell metabolism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody raised against N-terminal region (1-253 aa) of human TSHR can detect glycosylated subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Theranostics; Obatoclax and LY3009120 Efficiently Overcome Vemurafenib Resistance in Differentiated Thyroid Cancer.; Authors - Wei-Jun Wei
human	IF	Int J Nanomedicine; A TSHR-Targeting Aptamer in Monocytes Correlating with Clinical Activity in TAO; Authors - Jiamin Cao
mouse	IHC	Oxid Med Cell Longev; TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells.; Authors - Jingwen Fan
zebrafish	WB	Environ Pollut; Acute exposure of zebrafish embryo (Danio rerio) to flutolanil reveals its developmental mechanism of toxicity via disrupting the thyroid system and metabolism.; Authors - Miaomiao Teng
human	IF	Cancers (Basel); Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.; Authors - Luc H J Sondorp
human	WB	Front Endocrinol (Lausanne); Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression.; Authors - Dilidaer Muhanhali