TSN Polyclonal antibody, PBS Only

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  WB analysis of Jurkat using 55426-1-AP (same clone as 55426-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55426-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 55426-1-AP (same clone as 55426-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55426-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55426-1-AP (same clone as 55426-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55426-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 55426-1-AP (same clone as 55426-1-PBS)

  IP result of anti-TSN (IP:55426-1-AP, 3ug; Detection:55426-1-AP 1:300) with MCF-7 cells lysate 1600ug. This data was developed using the same antibody clone with 55426-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 55426-1-AP (same clone as 55426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55426-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 55426-1-AP (same clone as 55426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55426-1-PBS in a different storage buffer formulation.

TSN Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 55426-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

55426-1-PBS targets TSN in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: translin
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM_004622
• Gene Symbol: TSN
• Gene ID (NCBI): 7247
• RRID: AB_2881326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15631
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TSN, also named as Translin or Component 3 of promoter of RISC, is a 228 amino acid protein, which belongs to the translin family. TSN as a DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translations, mostly is involved in immunoglobulin T-cell receptor gene segments. TSN May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.