TSN Polyclonal antibody

TSN Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55426-1-AP

Synonyms

REHF 1, translin, TRSLN, TSN

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Validation Data Gallery

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  WB analysis of Jurkat using 55426-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55426-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55426-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 55426-1-AP

  IP result of anti-TSN (IP:55426-1-AP, 3ug; Detection:55426-1-AP 1:300) with MCF-7 cells lysate 1600ug.

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  IHC staining of mouse testis using 55426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55426-1-AP targets TSN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: translin
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM_004622
• Gene Symbol: TSN
• Gene ID (NCBI): 7247
• RRID: AB_2881326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSN, also named as Translin or Component 3 of promoter of RISC, is a 228 amino acid protein, which belongs to the translin family. TSN as a DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translations, mostly is involved in immunoglobulin T-cell receptor gene segments. TSN May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.