TSPAN5 Polyclonal antibody, PBS Only

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  WB analysis of Jurkat using 12122-1-AP (same clone as 12122-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12122-1-AP (TSPAN5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12122-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 12122-1-AP (same clone as 12122-1-PBS)

  WB result of TSPAN5 antibody (12122-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSPAN5 transfected Jurkat cells. This data was developed using the same antibody clone with 12122-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 12122-1-AP (same clone as 12122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12122-1-AP (TSPAN5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 12122-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TSPAN5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12122-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Tspan-5, Transmembrane 4 superfamily member 9, TM4SF9, Tetraspanin-5, Tetraspan NET-4

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Product Information

12122-1-PBS targets TSPAN5 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN5 fusion protein Ag2765
• Full Name: tetraspanin 5
• Calculated Molecular Weight: 268 aa, 30 kDa
• Observed Molecular Weight: 30-40 kDa, 90 kDa
• GenBank Accession Number: BC009704
• Gene Symbol: TSPAN5
• Gene ID (NCBI): 10098
• RRID: AB_10638611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62079
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TSPAN5 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN5 belongs to the TSPANC8 subfamily, which also include TSPAN10, TSPAN14, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).