TSPAN5 Polyclonal antibody

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• KD/KO Validated

TSPAN5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12122-1-AP

Synonyms

Tspan-5, Transmembrane 4 superfamily member 9, TM4SF9, Tetraspanin-5, Tetraspan NET-4

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Validation Data Gallery

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  WB analysis of Jurkat using 12122-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12122-1-AP (TSPAN5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12122-1-AP

  WB result of TSPAN5 antibody (12122-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSPAN5 transfected Jurkat cells.

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  IHC staining of human stomach cancer using 12122-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12122-1-AP (TSPAN5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12122-1-AP targets TSPAN5 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN5 fusion protein Ag2765
• Full Name: tetraspanin 5
• Calculated Molecular Weight: 268 aa, 30 kDa
• Observed Molecular Weight: 30-40 kDa, 90 kDa
• GenBank Accession Number: BC009704
• Gene Symbol: TSPAN5
• Gene ID (NCBI): 10098
• RRID: AB_10638611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN5 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN5 belongs to the TSPANC8 subfamily, which also include TSPAN10, TSPAN14, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

Publications

Species	Application	Title
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj
human	WB	Heliyon; Susceptibility of TSPAN5 polymorphisms to Kawasaki disease and immunoglobulin therapy resistance in southern Chinese children; Authors - Kaining Chen