TTC9C Recombinant antibody

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  WB analysis using 84611-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84611-2-RR (TTC9C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84611-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84611-2-RR (TTC9C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 84611-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84611-2-RR (TTC9C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84611-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84611-2-RR against Human TTC9C were performed. The affinity constant is below 1 pM.

TTC9C Recombinant Antibody for WB, IHC, ELISA

Cat No. 84611-2-RR

Clone No.242061D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

TPR repeat protein 9C, Tetratricopeptide repeat protein 9C, 242061D12

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Tested Applications

• Positive WB detected in: THP-1 cells, mouse thymus tissue, mouse skin tissue, mouse stomach tissue, human kidney tissue, HeLa cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84611-2-RR targets TTC9C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TTC9C fusion protein Ag37081
• Full Name: tetratricopeptide repeat domain 9C
• Observed Molecular Weight: 19-22 kDa
• GenBank Accession Number: BC032123
• Gene Symbol: TTC9C
• Gene ID (NCBI): 283237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N5M4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTC9 family belongs to tetratricopeptide repeat (TPR)-containing proteins family (PMID: 35949301).The TPR motifs are arranged in antiparallel α-helical hairpins which are clustered to form flexible grooved interfaces. Members in TRP-containing proteins family are involved in a diverse range of biological functions, including cell cycle control, transcription, protein folding, and steroid receptor signaling (PMID: 15497498;PMID: 10786835).