TTC9C Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84611-2-RR (same clone as 84611-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84611-2-RR (TTC9C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84611-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 84611-2-RR (same clone as 84611-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84611-2-RR (TTC9C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84611-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84611-2-RR (same clone as 84611-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84611-2-RR (TTC9C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84611-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01448-1

  Cytometric bead array standard curve of MP01448-1, TTC9C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84611-2-PBS. Detection antibody: 84611-1-PBS. Standard: Ag37081. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84611-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84611-2-RR against Human TTC9C were performed. The affinity constant is below 1 pM.

TTC9C Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 84611-2-PBS

Clone No.242061D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

TPR repeat protein 9C, Tetratricopeptide repeat protein 9C, 242061D12

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Product Information

84611-2-PBS targets TTC9C as part of a matched antibody pair:

MP01448-1: 84611-2-PBS capture and 84611-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TTC9C fusion protein Ag37081
• Full Name: tetratricopeptide repeat domain 9C
• Observed Molecular Weight: 19-22 kDa
• GenBank Accession Number: BC032123
• Gene Symbol: TTC9C
• Gene ID (NCBI): 283237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N5M4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TTC9 family belongs to tetratricopeptide repeat (TPR)-containing proteins family (PMID: 35949301).The TPR motifs are arranged in antiparallel α-helical hairpins which are clustered to form flexible grooved interfaces. Members in TRP-containing proteins family are involved in a diverse range of biological functions, including cell cycle control, transcription, protein folding, and steroid receptor signaling (PMID: 15497498;PMID: 10786835).