TXNRD2 Polyclonal antibody

• 

  WB analysis using 16360-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16360-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16360-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16360-1-AP (TXNRD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16360-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16360-1-AP (TXNRD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16360-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16360-1-AP (TXNRD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TXNRD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16360-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

KIAA1652, Selenoprotein Z, SELZ, thioredoxin reductase 2, Thioredoxin reductase TR3, TR, TR BETA, TR3, TRXR2, TXNRD2

---

Tested Applications

• Positive WB detected in: HepG2 cells, rat liver tissue, MCF-7 cells, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16360-1-AP targets TXNRD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8367

  Product name: Recombinant human TXNRD2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 137-485 aa of BC007489

  Sequence: EILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCUG

• Full Name: thioredoxin reductase 2
• Calculated Molecular Weight: 524 aa, 56 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC007489
• Gene Symbol: TXNRD2
• Gene ID (NCBI): 10587
• RRID: AB_2210258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NNW7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human mitochondrial thioredoxin reductase (TXNRD2) is a selenocysteine-containing enzyme essential for mitochondrial oxygen radical scavenging. Three mammalian thioredoxin reductases exist; a cytosolic (TXNRD1), a mitochondrial (TXNRD2), and a testis-specific thioredoxin reductase (TXNRD3). (PMID: 21247928, PMID: 26199228)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	WB	Nat Chem Biol; Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.; Authors - Zhipeng Li
rat	WB,IF	Oxid Med Cell Longev; Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics.; Authors - Xinwei Yang
mouse	WB	Antioxidants (Basel); Selenium Status in Diet Affects Nephrotoxicity Induced by Cisplatin in Mice.; Authors - Shuang Liu
mouse	WB	J Biol Chem; Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice.; Authors - Huafeng Wang
mouse	WB	Sci Rep; Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.; Authors - B Chellan