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Thymidylate synthase Polyclonal antibody
Thymidylate synthase Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, ELISA
Cat no : 15047-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, HEK-293T cells, NIH/3T3 cells, MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human endometrial cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 17 publications below|
|IHC||See 5 publications below|
15047-1-AP targets Thymidylate synthase in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Thymidylate synthase fusion protein Ag7027|
|Full Name||thymidylate synthetase|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||32-36 kDa|
|GenBank accession number||BC002567|
|Gene ID (NCBI)||7298|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.
|Product Specific Protocols|
|WB protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|IHC protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|IF protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|IP protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|FC protocol for Thymidylate synthase antibody 15047-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Pineal Res
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.
5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.
The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.
Cell Chem Biol
CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.
Comprehensive serum metabolomics and network analysis to reveal the mechanism of gypenosides in treating lung cancer and enhancing the pharmacological effects of cisplatin