Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody

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  FC experiment of RAW 264.7 using 98114-1-RR

  1x10^6 untreated or LPS-treated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody (98114-1-RR, Clone: 240875G1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of RAW 264.7 using 98114-1-RR

  1x10^6 LPS-treated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody (98114-1-RR, Clone: 240875G1) (red) or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody (98114-1-RR, Clone: 240875G1) (black, dashed) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98114-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98114-1-RR against Mouse Tissue Factor/CD142 were performed. The affinity constant is 0.442 nM.

Tissue Factor/CD142 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98114-1-RR

Clone No.240875G1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

F3, Coagulation factor III, CD142, Tissue factor, TFA

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Tested Applications

• Positive FC detected in: LPS treated RAW 264.7 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98114-1-RR targets Tissue Factor/CD142 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: coagulation factor III
• Calculated Molecular Weight: 33kDa
• Gene Symbol: Tissue Factor
• Gene ID (NCBI): 14066
• RRID: AB_3672261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P20352
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named CD142, coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).