|Positive WB detected in||HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, SKOV-3 cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10105-2-AP targets UBE2T/HSPC150 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||UBE2T/HSPC150 fusion protein Ag0153|
|Full Name||ubiquitin-conjugating enzyme E2T (putative)|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC004152|
|Gene ID (NCBI)||29089|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
Cancer Manag Res
UBE2T promotes proliferation via G2/M checkpoint in hepatocellular carcinoma.
Amplification and overexpression of E2 ubiquitin conjugase UBE2T promotes homologous recombination in multiple myeloma.
J Exp Clin Cancer Res
UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.
Expression of ubiquitin-conjugating enzyme E2T in colorectal cancers and clinical implications.
Elevated expression of UBE2T in lung cancer tumors and cell lines.