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  • KD/KO Validated

UBE2T/HSPC150 Polyclonal antibody

UBE2T/HSPC150 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 10105-2-AP


HSPC150, PIG50, UBE2T, UBE2T/HSPC150, Ubiquitin carrier protein T, Ubiquitin protein ligase T

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, Jurkat cells, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10105-2-AP targets UBE2T/HSPC150 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2T/HSPC150 fusion protein Ag0153
Full Name ubiquitin-conjugating enzyme E2T (putative)
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC004152
Gene Symbol UBE2T
Gene ID (NCBI) 29089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.


Product Specific Protocols
WB protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
IF protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
IP protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Am J Hum Genet

Mutations in the Gene Encoding the E2 Conjugating Enzyme UBE2T Cause Fanconi Anemia.

Authors - Asuka Hira

Cancer Res

Proteasome function is required for DNA damage response and fanconi anemia pathway activation.

Authors - Céline Jacquemont

Blood Adv

Amplification and overexpression of E2 ubiquitin conjugase UBE2T promotes homologous recombination in multiple myeloma.

Authors - David A Alagpulinsa


UBE2T promotes nasopharyngeal carcinoma cell proliferation, invasion, and metastasis by activating the AKT/GSK3β/β-catenin pathway.

Authors - Wei Hu
  • KD Validated

Biomed Pharmacother

Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.

Authors - Shuai Huang

Tumour Biol

Ubiquitin-Conjugating Enzyme E2T is an Independent Prognostic Factor and Promotes Gastric Cancer Progression.

Authors - Hao Yu