• Featured Product
  • KD/KO Validated

UBE2T/HSPC150 Polyclonal antibody

UBE2T/HSPC150 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10105-2-AP

Synonyms

HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, Jurkat cells, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10105-2-AP targets UBE2T/HSPC150 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2T/HSPC150 fusion protein Ag0153
Full Name ubiquitin-conjugating enzyme E2T (putative)
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC004152
Gene symbol UBE2T
Gene ID (NCBI) 29089
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Protocols

Product Specific Protocols
WB protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
IF protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
IP protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

JCI Insight

PMID: 31292296

Authors - Isabelle K Vila
humanWB

Cancer Manag Res

UBE2T promotes proliferation via G2/M checkpoint in hepatocellular carcinoma.

Authors - Li-Li Liu
  • KD Validated
humanWB

Blood Adv

Amplification and overexpression of E2 ubiquitin conjugase UBE2T promotes homologous recombination in multiple myeloma.

Authors - David A Alagpulinsa
humanWB,IHC,IF

J Exp Clin Cancer Res

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Authors - Jingyuan Sun
  • KD Validated
humanWB,IHC

Oncol Lett

Expression of ubiquitin-conjugating enzyme E2T in colorectal cancers and clinical implications.

Authors - Xiangtian Wu
humanWB,IHC

Tumour Biol

Elevated expression of UBE2T in lung cancer tumors and cell lines.

Authors - Hao Jia J