UBF1 Polyclonal antibody, PBS Only

• 

  WB analysis of HT-1080 using 20660-1-AP (same clone as 20660-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20660-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 20660-1-AP (same clone as 20660-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20660-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20660-1-AP (same clone as 20660-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20660-1-PBS in a different storage buffer formulation.

UBF1 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 20660-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

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Product Information

20660-1-PBS targets UBF1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: upstream binding transcription factor, RNA polymerase I
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: NM_014233
• Gene Symbol: UBF1
• Gene ID (NCBI): 7343
• RRID: AB_2878718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17480
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)