UBF1 Polyclonal antibody

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• UBF1 Polyclonal antibody (20660-1-AP)

• 

  WB analysis of HT-1080 using 20660-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20660-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20660-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

UBF1 Polyclonal Antibody for WB, ELISA

Cat No. 20660-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UBTF, UBF-1, UBF 1, UBF, NOR 90

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Tested Applications

• Positive WB detected in: HT-1080 cells, HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

20660-1-AP targets UBF1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: upstream binding transcription factor, RNA polymerase I
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: NM_014233
• Gene Symbol: UBF1
• Gene ID (NCBI): 7343
• RRID: AB_2878718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17480
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Rep; M2 macrophages and tumor cells engage in a metabolic feedback loop to drive HCC progression.; Authors - Yimin Nong
human	WB	Int J Mol Sci; Pancancer Analysis and the Oncogenic Role of UBTF in Breast Invasive Carcinoma.; Authors - Mingang He ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Detectable but weak signal in the nucleoli

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells

UBF1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:20660-1-AP)