UBF1 Polyclonal antibody

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• UBF1 Polyclonal antibody (20660-1-AP)

UBF1 Polyclonal Antibody for WB, ELISA

Cat No. 20660-1-AP Copied!

Publications (2)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UBTF, UBF-1, UBF 1, UBF, NOR 90

• 

  WB analysis of HT-1080 using 20660-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 20660-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 20660-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HT-1080 cells, HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20660-1-AP targets UBF1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: upstream binding transcription factor, RNA polymerase I
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: NM_014233
• Gene Symbol: UBF1
• Gene ID (NCBI): 7343
• RRID: AB_2878718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17480
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)

Publications

What published studies show

The UBF1 Polyclonal antibody (Cat# 20660-1-AP) has 2 citations. These appear in International Journal of Molecular Sciences and Scientific Reports. The cited research spans oncology fields: one study investigates the oncogenic role of UBTF in breast invasive carcinoma via pancancer analysis, while the other examines how M2 macrophages and tumor cells form a metabolic feedback loop driving hepatocellular carcinoma (HCC) progression. Both citations use the antibody for Western blot in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Mol Sci; Pancancer Analysis and the Oncogenic Role of UBTF in Breast Invasive Carcinoma.; Authors - Mingang He ; KD Validated
human	WB	Sci Rep; M2 macrophages and tumor cells engage in a metabolic feedback loop to drive HCC progression.; Authors - Yimin Nong

Reviews

3.0 out of 5

1 verified customer reviews

What customers say

The single review for this antibody (used in immunofluorescence at 1:1000 on HeLa cells) rated it 3 stars. The reviewer reported that the signal in the nucleoli was detectable but weak. Overall, the feedback suggests the antibody works but may require optimization for stronger signal.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Tatyana (Verified Customer) (12-04-2023)

Detectable but weak signal in the nucleoli

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells

UBF1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:20660-1-AP)