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UCHL1/PGP9.5 Polyclonal antibody
UCHL1/PGP9.5 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, CoIP,ELISA
Conjugate
Unconjugated
Cat no : 14730-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, C6 cells, NCI-H1299 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A549 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 14 publications below |
| IHC | See 6 publications below |
| IF | See 24 publications below |
| ELISA | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
14730-1-AP targets UCHL1/PGP9.5 in WB, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey, mouse, Non-human primate, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UCHL1/PGP9.5 fusion protein Ag6490 |
| Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC000332 |
| Gene symbol | UCHL1 |
| Gene ID (NCBI) | 7345 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UCHL1/PGP9.5 antibody 14730-1-AP | Download protocol |
| IHC protocol for UCHL1/PGP9.5 antibody 14730-1-AP | Download protocol |
| IF protocol for UCHL1/PGP9.5 antibody 14730-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Neurosci Possible involvement of peripheral TRP channels in the hydrogen sulfide-induced hyperalgesia in diabetic rats. | ||
Aging (Albany NY) Aging-dependent decrease in the numbers of enteric neurons, interstitial cells of Cajal and expression of connexin43 in various regions of gastrointestinal tract. | ||
Sci Rep Mitochondrial uncoupling has no effect on microvascular complications in type 2 diabetes. | ||
Cell Rep Aryl Hydrocarbon Receptor Interacting Protein Maintains Germinal Center B Cells through Suppression of BCL6 Degradation. | ||
Ann Clin Transl Neurol Absence of UCHL 1 function leads to selective motor neuropathy.
| ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kathryn (Verified Customer) (07-10-2020) | We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).
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FH Ula (Verified Customer) (11-11-2019) | The antibody produced beautiful staining in mouse skin treated with PLP fixative.
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FH Rachel (Verified Customer) (07-03-2019) | This product binds with high frequency and sensitivity. I would recommend it to any researchers.
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