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- KD/KO Validated
UCHL1/PGP9.5 Monoclonal antibody
UCHL1/PGP9.5 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 66230-1-Ig
Validation Data Gallery
|Positive WB detected in
|Neuro-2a cells, Y79 cells, PC-12 cells, Pig brain tissue, Pig cerebellum tissue, Rat brain tissue, Rat cerebellum tissue, Mouse brain tissue, Mouse cerebellum tissue
|Positive IHC detected in
|mouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:20000-1:100000
|IHC : 1:4000-1:16000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66230-1-Ig targets UCHL1/PGP9.5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|human, mouse, pig, rat
|human, mouse, rat, pig
|Host / Isotype
|Mouse / IgG1
|UCHL1/PGP9.5 fusion protein Ag6547
|ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)
|Product Specific Protocols
|WB protocol for UCHL1/PGP9.5 antibody 66230-1-Ig
|IHC protocol for UCHL1/PGP9.5 antibody 66230-1-Ig
|IF protocol for UCHL1/PGP9.5 antibody 66230-1-Ig
|FC protocol for UCHL1/PGP9.5 antibody 66230-1-Ig
|Click here to view our Standard Protocols
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