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UCHL1/PGP9.5 Monoclonal antibody

UCHL1/PGP9.5 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, pig, rat







Cat no : 66230-1-Ig


Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCH-L1, UCHL1/PGP9.5

Tested Applications

Positive WB detected inNeuro-2a cells, Y79 cells, PC-12 cells, Pig brain tissue, Pig cerebellum tissue, Rat brain tissue, Rat cerebellum tissue, Mouse brain tissue, Mouse cerebellum tissue
Positive IHC detected inmouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inY79 cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66230-1-Ig targets UCHL1/PGP9.5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UCHL1/PGP9.5 fusion protein Ag6547
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated molecular weight 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC000332
Gene symbol UCHL1
Gene ID (NCBI) 7345
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)


Product Specific Protocols
WB protocol for UCHL1/PGP9.5 antibody 66230-1-IgDownload protocol
IHC protocol for UCHL1/PGP9.5 antibody 66230-1-IgDownload protocol
IF protocol for UCHL1/PGP9.5 antibody 66230-1-IgDownload protocol
FC protocol for UCHL1/PGP9.5 antibody 66230-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




UCH-L1-mediated Down-regulation of Estrogen Receptor α Contributes to Insensitivity to Endocrine Therapy for Breast Cancer.

Authors - Xi-Sha Chen
  • KD Validated

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Isolation and in vitro expansion of porcine spermatogonial stem cells.

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ORL J Otorhinolaryngol Relat Spec

Role of the Ubiquitin C-Terminal Hydrolase L1-Modulated Ubiquitin Proteasome System in Auditory Cortex Senescence.

Authors - Ya Zhang

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Oxaliplatin-induced peripheral neuropathy can be minimized by pressurized regional intravascular delivery in an orthotopic murine pancreatic cancer model.

Authors - Jayanth Surya Narayanan Shankara Narayanan

PLoS Biol

The RSK2-RPS6 axis promotes axonal regeneration in the peripheral and central nervous systems

Authors - Charlotte Decourt