CoraLite® Plus 488-conjugated UCHL1/PGP9.5 Monoclonal antibody

UCHL1/PGP9.5 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9E11

Cat no : CL488-66230

Synonyms

Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCHL1/PGP9.5

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-66230

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 UCHL1/PGP9.5 antibody (CL488-66230, Clone: 1C9E11 ) at dilution of 1:200.

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  FC experiment of Y79 using CL488-66230

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UCHL1/PGP9.5 (CL488-66230, Clone:1C9E11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse brain tissue
• Positive FC detected in: Y79 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66230 targets UCHL1/PGP9.5 in IF, FC (Intra) applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UCHL1/PGP9.5 fusion protein Ag6547
• Full Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC000332
• Gene Symbol: UCHL1
• Gene ID (NCBI): 7345
• RRID: AB_2883290
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C