UFM1 Recombinant antibody

• 

  WB analysis using 85068-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85068-5-RR (UFM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

UFM1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85068-5-RR

Clone No.242505E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Ubiquitin-fold modifier 1, ubiquitin fold modifier 1, C13orf20, BM-002, BM 002

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HuH-7 cells, HEK-293T cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85068-5-RR targets UFM1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UFM1 fusion protein Ag8667
• Full Name: ubiquitin-fold modifier 1
• Calculated Molecular Weight: 85 aa, 9 kDa
• Observed Molecular Weight: 9-12 kDa
• GenBank Accession Number: BC005193
• Gene Symbol: UFM1
• Gene ID (NCBI): 51569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61960
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UFM1 (Ubiquitin-fold modifier 1) is a ubiquitin-like protein covalently conjugated with intracellular proteins through UFMylation (modification by UFM1), a process similar to ubiquitylation (PMID: 38141606). UFM1 is conjugated to its target proteins by E1-like activating enzyme UBE1DC1 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation (PMID: 28234446; 33066455). At the molecular level, UFMylation is an important mediator of the protein function. Dysregulation of the UFM1 system, e.g., the knockout of UFMylation components, disturbs proteome homeostasis and triggers endoplasmic reticulum stress. Such changes are linked to developmental disorders, tumorigenesis, tissue injury, inflammation, and several hereditary neurological syndromes (PMID: 36932998).