UFM1 Recombinant antibody, PBS Only

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  WB analysis using 85068-5-RR (same clone as 85068-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85068-5-RR (UFM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85068-5-PBS in a different storage buffer formulation.

UFM1 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85068-5-PBS

Clone No.242505E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Ubiquitin-fold modifier 1, ubiquitin fold modifier 1, C13orf20, BM-002, BM 002

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Product Information

85068-5-PBS targets UFM1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UFM1 fusion protein Ag8667
• Full Name: ubiquitin-fold modifier 1
• Calculated Molecular Weight: 85 aa, 9 kDa
• Observed Molecular Weight: 9-12 kDa
• GenBank Accession Number: BC005193
• Gene Symbol: UFM1
• Gene ID (NCBI): 51569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61960
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UFM1 (Ubiquitin-fold modifier 1) is a ubiquitin-like protein covalently conjugated with intracellular proteins through UFMylation (modification by UFM1), a process similar to ubiquitylation (PMID: 38141606). UFM1 is conjugated to its target proteins by E1-like activating enzyme UBE1DC1 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation (PMID: 28234446; 33066455). At the molecular level, UFMylation is an important mediator of the protein function. Dysregulation of the UFM1 system, e.g., the knockout of UFMylation components, disturbs proteome homeostasis and triggers endoplasmic reticulum stress. Such changes are linked to developmental disorders, tumorigenesis, tissue injury, inflammation, and several hereditary neurological syndromes (PMID: 36932998).