UQCRC1 Polyclonal antibody

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  WB analysis using 21705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21705-1-AP

  WB result of UQCRC1 antibody (21705-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UQCRC1 transfected HeLa cells.

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  WB analysis of HeLa using 21705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

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  WB analysis of human brain using 21705-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 21705-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21705-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21705-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21705-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse large intestine using 21705-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21705-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21705-1-AP

  IP result of anti-UQCRC1 (IP:21705-1-AP, 4ug; Detection:21705-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human colon using 21705-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21705-1-AP (UQCRC1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21705-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21705-1-AP (UQCRC1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21705-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UQCRC1 antibody (21705-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

UQCRC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21705-1-AP

Publications(41)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Ubiquinol-cytochrome-c reductase complex core protein 1, QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, human heart tissue, mouse large intestine tissue, mouse kidney tissue, mouse spleen tissue, L02 cells, mouse liver tissue, rat liver tissue, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human normal colon, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 37 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

21705-1-AP targets UQCRC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UQCRC1 fusion protein Ag16322
• Full Name: ubiquinol-cytochrome c reductase core protein I
• Calculated Molecular Weight: 480 aa, 53 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC009586
• Gene Symbol: UQCRC1
• Gene ID (NCBI): 7384
• RRID: AB_10734437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31930
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

Publications

Species	Application	Title
human	WB	Sci Bull (Beijing); Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects; Authors - Shuixia Tan
mouse	WB	Biomaterials; Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.; Authors - Xiao-Tao He
human	IHC	Theranostics; Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.; Authors - Qing Wang
		Acta Pharmacol Sin; MicroRNA-214 contributes to Ang II-induced cardiac hypertrophy by targeting SIRT3 to provoke mitochondrial malfunction.; Authors - Yan-Qing Ding
human	WB	Int J Nanomedicine; Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect; Authors - Wenkai Chen
human	WB	Aging (Albany NY); Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism.; Authors - Delong Yin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xingyuan (Verified Customer) (06-30-2023)

Quality was alright, had non-specific band under it.

• Applications: Western Blot
• Cell Tissue Type: 293T

UQCRC1 Antibody Western Blot validation ( dilution) in 293T (Cat no:21705-1-AP)

FH

Xingyuan (Verified Customer) (06-30-2023)

Quality was alright, had non-specific band under it.

• Applications: Western Blot
• Cell Tissue Type: 293T