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UQCRC1 Polyclonal antibody
UQCRC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21705-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse brain tissue, mouse skeletal muscle tissue, human heart tissue, L02 cells, mouse large intestine tissue, MCF-7 cells, mouse kidney tissue, HepG2 cells, human brain tissue, human skeletal muscle tissue, mouse spleen tissue, mouse liver tissue, rat liver tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 23 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
The immunogen of 21705-1-AP is UQCRC1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | UQCRC1 fusion protein Ag16322 |
Full Name | ubiquinol-cytochrome c reductase core protein I |
Calculated molecular weight | 480 aa, 53 kDa |
Observed molecular weight | 45 kDa |
GenBank accession number | BC009586 |
Gene symbol | UQCRC1 |
Gene ID (NCBI) | 7384 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.
Protocols
Product Specific Protocols | |
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WB protocol for UQCRC1 antibody 21705-1-AP | Download protocol |
IHC protocol for UQCRC1 antibody 21705-1-AP | Download protocol |
IF protocol for UQCRC1 antibody 21705-1-AP | Download protocol |
IP protocol for UQCRC1 antibody 21705-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomaterials Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation. | ||
Theranostics Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer. | ||
Int J Nanomedicine Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect | ||
Cell Death Dis Avenanthramide A triggers potent ROS-mediated anti-tumor effects in colorectal cancer by directly targeting DDX3. | ||
Sci Rep Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts. | ||
J Biol Chem An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function. |