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UQCRC1 Polyclonal antibody

UQCRC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21705-1-AP

Synonyms

Complex III subunit 1, Core protein I, D3S3191, QCR1, UQCR1, UQCRC1



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse skeletal muscle tissue, human heart tissue, L02 cells, mouse large intestine tissue, MCF-7 cells, mouse kidney tissue, HepG2 cells, human brain tissue, human skeletal muscle tissue, mouse spleen tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21705-1-AP is UQCRC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCRC1 fusion protein Ag16322
Full Name ubiquinol-cytochrome c reductase core protein I
Calculated molecular weight 480 aa, 53 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC009586
Gene symbol UQCRC1
Gene ID (NCBI) 7384
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

Protocols

Product Specific Protocols
WB protocol for UQCRC1 antibody 21705-1-APDownload protocol
IHC protocol for UQCRC1 antibody 21705-1-APDownload protocol
IF protocol for UQCRC1 antibody 21705-1-APDownload protocol
IP protocol for UQCRC1 antibody 21705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biomaterials

Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.

Authors - Xiao-Tao He
humanIHC

Theranostics

Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.

Authors - Qing Wang
humanWB

Int J Nanomedicine

Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect

Authors - Wenkai Chen
humanWB

Cell Death Dis

Avenanthramide A triggers potent ROS-mediated anti-tumor effects in colorectal cancer by directly targeting DDX3.

Authors - Rong Fu
mouseWB

Sci Rep

Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts.

Authors - Huihui Li
humanWB

J Biol Chem

An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.

Authors - Xiaofen Jin